این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۱۴، شماره ۱، صفحات ۱۰۶-۱۱۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China
چکیده انگلیسی مقاله Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents’ brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | XinLei WANG
College of Basic Medicine, Dali University, Dali, Yunnan Province, China Jinci College of Shanxi Medical University, Taiyuan, Shanxi Province, China


| Ling DONG
College of Basic Medicine, Dali University, Dali, Yunnan Province, China


| Li ZHANG
College of Basic Medicine, Dali University, Dali, Yunnan Province, China


| Yan LV
College of Basic Medicine, Dali University, Dali, Yunnan Province, China


| HaiLong LI
College of Basic Medicine, Dali University, Dali, Yunnan Province, China
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1755
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-1353735.pdf
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات