این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Parasitology، جلد ۱۳، شماره ۳، صفحات ۴۷۳-۴۷۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Allelic Variations of Plasmodium vivax Apical Membrane Antigen-1 (Pv AMA-1) in Malarious Areas of Southeastern Iran Using PCR-RFLP Technique
چکیده انگلیسی مقاله Background: Although Plasmodium vivax is usually known as benign malaria, some variations of the parasite can result in acute and sever infection. In this study we tried to determine some genetic variations in PvAMA-1 antigen among the samples were collected form southeastern Iran. Methods: About two ml blood samples were collected into EDTA pre-dosed tubes from 30 P. vivax–infected patients individually between 2011 and 2013. A Giemsa stained thick and thin blood film was prepared from each of the patients. A PCR-RFLP technique was employed using EcoR-1, Pvu-II and Hind3 restriction enzymes to determine the allelic variations of the antigen. Results: A 1300bp gene corresponding to PvAMA-1 was selected for the amplification process. Among the total cases identified in this study 90% showed similar bounds when exposed to the restriction enzymes. Nine isolates (accession numbers: KF435081-KF435083 and JF682785-JF682790) were identified and registered in Gene bank. Identity among isolates was more than 96% in nucleotide level. Dendrogram clarified a close relationship among the clusters in spite of geographical distribution of the parasite. Conclusion: This study increased our data about prevalence and variation of PvAMA-1 alleles amongst P. vivax isolates in southeastern parts of Iran where besides native population bears considerable Afghan and Pakistani immigrants.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Afsaneh MOTEVALLI HAGHI
Dept. of Medical Parasitology and Mycology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran


| Sepide MORADI
Dept. of Medical Parasitology and Mycology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran


| Mehdi NATEGHPOUR
Dept. of Medical Parasitology and Mycology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran . Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran


| Gholamhossein EDRISSIAN
Dept. of Medical Parasitology and Mycology, School of Public Health, International Campus, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1285
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-1353770.pdf
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات