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Iranian Journal of Parasitology، جلد ۱۲، شماره ۲، صفحات ۲۵۱-۲۵۹
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عنوان انگلیسی Considerable Genetic Diversity of Trichomonas vaginalis Clinical Isolates in a Targeted Population in South of Iran
چکیده انگلیسی مقاله Background: The present study aimed to characterize genetically and to compare the most frequently occurring strains of Trichomonas vaginalis isolated from southern Iran.Methods: Totally, 150 vaginal swab and urine specimens were collected from symptomatic and asymptomatic women from May 2012 to Jun 2013.This study implemented a sensitive and reliable PCR-restriction fragment length polymorphism (RFLP) typing method on the actin gene. Moreover, one representative sample of each identified genotype was subjected to sequencing.Results: Twenty-four T. vaginalis isolates were positive and6 distinct electrophoretic patterns (H, E, G, I, M, N) were identified. Genotypes H and I were found to be more prevalent (50 and 37.5%) in Kerman and Shiraz, respectively. The phylogenetic analysis showed that two isolates were located as a separated clade with the other T. vaginalis isolates.Conclusion: The obtained findings showed a considerable genetic polymorphism of clinical isolates from the population studied. More studies may be warranted in future as to unveiling any possible links between a given genotype/cluster and pathogenic behavior of T. vaginalis.
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نویسندگان مقاله | Razieh TAVAKOLI OLIAEE
Dept. of Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran


| Zahra BABAEI
Dept. of Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran


| Gholam Reza HATAM
Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| Amir TAVAKOLI KARESHK
Dept. of Parasitology and Mycology, Kerman University of Medical Sciences, Kerman, Iran


| Hosein MAHMOUDVAND
Dept. of Medical Parasitology and Mycology, Lorestan University of Medical Sciences, Khorramabad, Iran


| Arghavan VAFAFAR
Dept. of Parasitology and Mycology, Shiraz University of Medical Sciences, Shiraz, Iran


| Naser ZIAALI
Research Center for Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1631
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-1353831.pdf
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