| چکیده انگلیسی مقاله |
Background:Natural killer (NK) cells play an important role in early stages of innate immune responses against viral and tumoral attacks. Activation of NK cells by leishmaniasis results in secretion of cytokines such as interferon (IFN)-γ and tumor necrosis factor (TNF)-α, which enhance the phagocytosis and clearance of parasite. Lipophosphoglycan 3 (LPG3), the Leishmania homologous with GRP94 (glucose regulated protein 94), a member of HSP90 family, contributes to LPG assembly as the most abundant macromolecule on the surface of Leishmania promastigotes. Methods:We purified NK cells from healthy individuals (n=10) using magnetic-activated cell sorting (MACS) technology. Purified NK cells were co-incubated with different concentrations of recombinant LPG3 (rLPG3), and its N-terminal (NT) and C-terminal (CT) fragments. Finally, the production of IFN-γ and TNF-α by NK cells were measured by ELISA. Results:Recombinant LPG3 but not its fragments (CT and NT), could significantly enhance the production of TNF-α by NK cells (P< 0.05). Moreover, rLPG3, CT, and NT fragments were markedly stimulated the secretion of IFN-γ by NK cells (P< 0.001). Conclusion: The Leishmania LPG3 antigen could effectively activate NK cells, in vitro. Leishmania LPG3 participates in the innate immunity against leishmaniasis and thereby improves the effective parasite destruction.However, its efficiency should be tested in vivo. |
| نویسندگان مقاله |
| Sanaz RASOLZADEH
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
| Mostafa HAJI FATAHALIHA
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
| Maryam HOSSEINI
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
| Reza JAFARI
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
| Abolfazl MIAHIPOUR
Dept. of Medical Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran
| Sevil SADREDDINI
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
| Zohreh BABALO
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
| Hossein SAMADI KAFIL
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
| Mehdi YOUSEFI
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Dept. of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
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