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Iranian Journal of Veterinary Research، جلد ۱۰، شماره ۱، صفحات ۶۱-۶۵
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عنوان انگلیسی Pituitary primary cell culture of Common carp (Cyprinus Carpio) and evaluation of its secretion effect on endocrine activity of incubated ovarian follicles
چکیده انگلیسی مقاله In this study, five carp pituitary glands were collected and dispersed enzymatically and mechanically.Then, the cells were cultivated as monolayer in MEM (minimum essential medium Eagle). The culture mediawere collected after 72 h and frozen at -20°C. Carp ovarian follicles also were separated mechanically andincubated in BSS (basic salt solution) Cortland medium in 24-well microplates for 48 h at 20°C. Then, theywere divided into two groups: control group which were incubated in BSS medium and experimental groupwhich subdivided into three subgroups according to treatment with different concentration of collectedpituitary secretion (50, 100 and 200 μl/ml). Follicles culture media were collected 24 h later and wereanalyzed for 17-β-oestradiol (E2) and 17-α-hydroxy progesterone (P4) content by radioimmunoassay (RIA).The results showed that adding low concentration (50 μl/ml) of collected pituitary secretion (CPS) increasedsteroid hormones (E2 and P4) secretion of incubated ovarian follicles significantly (P
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نویسندگان مقاله n سلامت |
department of basic sciences, faculty of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

n البوغبیش |
department of basic sciences, faculty of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

m هاشمی تبار |
department of anatomical sciences, school of medicine, jondishapoor university of medical sciences, ahvaz, iran

m مصباح |
department of aquatic animal health and diseases, faculty of veterinary medicine, shahid chamran university of ahvaz, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

a آهنگرپور |
department of physiology, school of medicine, jondishapoor university of medical sciences, ahvaz, iran

نشانی اینترنتی http://ijvr.shirazu.ac.ir/article_1091_450b7534083badba7259d42b3cd724a2.pdf
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