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Iranian Journal of Veterinary Research، جلد ۷، شماره ۱، صفحات ۱۷-۲۱
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عنوان انگلیسی Polymerase chain reaction for the detection and differentiation of Marek’s disease virus strains MDV-1 and HVT
چکیده انگلیسی مقاله Marek’s disease (MD) is a lymphoproliferative disease of chickens characterized by lymphocyticinfiltration of various organs. The present study was an attempt to use polymerase chain reaction (PCR) tooptimize a rapid and reliable assay for detection of MDV genome. Detection of serotype 1 of MDV (MDV-1)was confirmed by presence of a 200 bp DNA fragment as a PCR product. Differentiation of MDV-1 andherpesvirus of turkeys (HVT) was also conducted using specific primers from the glycoprotein A (gA) geneand a 388 bp DNA fragment was amplified from HVT genome. The specificity of the test was confirmed bysequencing of PCR products. Results indicate that MDV-1 can be diagnosed in clinical samples and inoculated cell cultures which is used for virus isolation. In addition, differentiation between MDV-1 and HVT viruses was confirmed based on the size of PCR products. The test proved to be rapid and reliable and can be performed as a robust diagnostic test in veterinary diagnostic laboratories.
کلیدواژه‌های انگلیسی مقاله Marek’s disease virus,PCR,Detection
نویسندگان مقاله محمدرضا صادقی | m r
national institute for genetic engineering and biotechnology, tehran, iran

سید علی قرشی | s a
national institute for genetic engineering and biotechnology, tehran, iran

r کارگر موخر | kargar moakhar
razi vaccine and serum research institute, karadj, iran
سازمان اصلی تایید شده: موسسه تحقیقات واکسن و سرم سازی رازی (Razi vaccine and serum research institute)

d مرشدی |
national institute for genetic engineering and biotechnology, tehran, iran

r صالحی تبار | salehi tabar
national institute for genetic engineering and biotechnology, tehran, iran

سید سعید قایم مقامی | s s
veterinary medicine research center, agriculture organization, markazi province, arak, iran

نشانی اینترنتی http://ijvr.shirazu.ac.ir/article_2675_04cdb1d922cc0ba55fd73aea7a1bb43e.pdf
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