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Medical Journal of Islamic Republic of Iran، جلد ۱۰، شماره ۴، صفحات ۲۹۹-۳۰۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی NOVEL REAGENTS FOR CREATINE KINASE
چکیده انگلیسی مقاله The purpose of this investigation was to develop a simple colorimetric method for creatine kinase (CK). The new method is based on the reaction of creatine, formed enzymatically from creatine phosphate and ADP, with different glyoxal compounds. Hydrated glyoxals, such as para-nitrophenyl, 2-thiophene, 4- biphenyl, 4, 4' -biphenyl,α-naphthyl, β-naphthyl, para-chlorophenyl, and styryl were synthesized and allowed to react with creatine. Among the glyoxals, the 2- thiophene derivative was the best in terms of the stability and intensity of the colored complex which was produced under mild alkaline conditions. The complex absorbed maximally at 460 nm with an extinction coefficient of 1.56x 1 04 M-I Cm-I. This reagent was used to determine CK in the sera of normal human beings and patients with myocardial infarction. The results obtained were in agreement with those obtained by another available method for CK. However, this new method is simple, less time consuming and employs a single reagent for color development. Such a simple method might be of value in clinical laboratories with little access to sophisticated instruments such as auto analyzers and spectrophotometers
کلیدواژه‌های انگلیسی مقاله Creatine kinase, reagents, colorimetry
نویسندگان مقاله محمود امین لاری | mahmoud aminlari
from the department of biochemistry, school of veterinary medicine


حبیب فیروزآبادی | habib firoozabadi
the department of chemistry, shiraz university, shiraz, islamic republic of iran.

سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

orli simozar | orli simozar
the department of chemistry, shiraz university, shiraz, islamic republic of iran.

سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1-416&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Biological Sciences
نوع مقاله منتشر شده Original Research: Basic Science in Medicine
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