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Medical Journal of Islamic Republic of Iran، جلد ۹، شماره ۱، صفحات ۳۷-۴۰
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عنوان انگلیسی LA BORATORY IDE NTIFICATION OF DERMATOPHYTES USING PROTOPLAST HYBRIDIZATION
چکیده انگلیسی مقاله In this study techniques for laboratory identification of dermatophyte fungi through protoplast hybridization were established. Firstly, auxotrophic mutants of different species of microsporum and trichophyton were induced and identified. Secondly, protoplasts from these mutants were isolated by digestion of their mycelium with Novozyme 234 using CaCI, (O.4M) as an osmotic stabilizer and glycine + HCI (pH 4.5) as the buffer system. Thirdly, isolated protoplasts from different species were fused using a solution containing 35% polyethylene glycol, I M KCI, 0.05 M CaCI, and 0.05 M glycine with pH 6.1. Afterwards, the fusion products were plated onto minimal and complete media. It was found that protoplasts from different auxotrophic mutants from the same species hybridized and complemented each other and grew on both minimal and complete media. whereas mutants from different species did not have the ability to complement each other and therefore grew only on complete media. Information obtained in this study may prove useful for definite identification of suspected species of dennatophytes other than morphological criteria in laboratories.
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نویسندگان مقاله مصطفی چادگانی پور | mostafa chadeganipour
from the amp;apos; dept. of mycology and parasitology, isfahan university of medical sciences, isfahan

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

vernon ahmadjian | vernon ahmadjian
the mycology laboratory, clark university, massachusetts, u.s.a.
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-554&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Parasitology and Mycology
نوع مقاله منتشر شده Original Research
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