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Iranian Journal of Medical Sciences، جلد ۴۳، شماره ۵، صفحات ۵۲۳-۵۳۲
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عنوان انگلیسی 9-cis-Retinoic Acid and 1,25-dihydroxy Vitamin D3 Improve the Differentiation of Neural Stem Cells into Oligodendrocytes through the Inhibition of the Notch and Wnt Signaling Pathways
چکیده انگلیسی مقاله Background: Differentiating oligodendrocyte precursor cells (OPCs) into oligodendrocytes could be improved by inhibiting signaling pathways such as Wnt and Notch. 9-cis-retinoic acid (9-cis-RA) and 1,25-dihydroxyvitamin D3 (1,25[OH]2D3) can ameliorate oligodendrogenesis. We investigated whether they could increase oligodendrogenesis by inhibiting the Wnt and Notch signaling pathways.Methods: Cortical neural stem cells were isolated from 14-day-old rat embryos and cultured using the neurosphere assay. The cells were treated in 4 different conditions for 1 week: the negative control group received only the basic fibroblast growth factor, the positive control group received only T3 without growth factors, the RA group was treated with 9-cis-RA, and the Vit D3 group was treated with 1,25(OH)2D3. The effects of 9-cis-RA and 1,25(OH)2D3 on the level of the myelin basic protein (MBP) and the gene expression of the SOX10, MBP gene, HES5, and LRP6 were studied using flow cytometry and real-time PCR. The data were analyzed using one-way ANOVA with GraphPad Prism. A P value less than 0.05 was considered significant. Results: The mRNA expressions of the SOX10, MBP, and MBP gene were significantly increased in the treated groups compared with the negative control group; the increase was similar in the 9-cis-RA group and the positive control group. Furthermore, 9-cis-RA significantly decreased the expression of the HES5 gene, a Notch signaling pathway transcription factor, and 1,25(OH)2D3 significantly reduced the expression of the LRP6 gene, a Wnt signaling pathway co-receptor. Conclusion: It seems that 9-cis-RA and 1,25(OH)2D3 are good candidates to improve the differentiation of OPCs into oligodendrocytes.
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نویسندگان مقاله Saeedeh Saeb |
Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran;

Hassan Azari |
Neural Stem Cell and Regenerative Neuroscience Laboratory, Department of Anatomical Sciences and Shiraz Stem Cell Institute, Shiraz University of Medical Sciences, Shiraz, Iran

Zohreh Mostafavi-Pour |
Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran;

Amir Ghanbari |
Department of Anatomical Sciences, Yasuj University of Medical sciences, Yasuj, Iran

Sepideh Ebrahimi |
Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran;

Pooneh Mokarram |
Department of Biochemistry, Shiraz University of Medical Sciences, Shiraz, Iran;

نشانی اینترنتی http://ijms.sums.ac.ir/article_40573_b201afc9f11c127222a9469d0166fc34.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/147/article-147-1611458.pdf
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