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Medical Journal of Islamic Republic of Iran، جلد ۶، شماره ۲، صفحات ۱۲۹-۱۳۳
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عنوان انگلیسی ALTERATIONS OF ADP-RIBOSYLATION AND DNA-BREAKS IN AGING BRAIN CELLS
چکیده انگلیسی مقاله Neuronal and astroglial cells were prepared from whole brain of three month and 30-month- old rats for study of alterations in the nuclear poly ADP-ribosylation and DNA breaks with age. The relative purity of the cell preparations was confirmed by the determination of the neurofilament (low molecular weight) and glutamine synthetase content of the cells using ELISA. An increase (75%) in the poly ADP-ribosylation was observed in the whole brain cell suspension of aged rats, whereas the increase was markedly pronounced (460%) when the reaction was measured in the purified neuronal preparations. The rate of poly ADP-ribosylation in the astroglial fractions prepared from aged rat brain was higher than that of adult levels (67%). An unexpectedly high increase of ADP-ribosylation in the neurons and a much lower rate in the astroglial cells was thus recorded. The amount of DNA breaks was also higher in the neuronal preparation in aged brain as compared to that of adult levels. The amount of DNA breaks was much lower in the astroglial cells and aging had no effect on DNA breaks of these cells. The close relationship between DNA breaks and poly ADP-ribosylation in the different cell types suggest that neurons are more susceptible to the metabolic alterations of the aging process
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نویسندگان مقاله منوچهر messripour• | manouchehr messripour•
the department of biochemistry, faculty of pharmacy, isfahan university of medical sciences, isfahan, islamic republic of iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

علی رستگار | ali rastegar
from the centre de neurochime du c.n.r.s., 5 rue blaise pascal, 67084 strasbourg cedex, france


lucien ciesielski | lucien ciesielski
from the centre de neurochime du c.n.r.s., 5 rue blaise pascal, 67084 strasbourg cedex, france


magali chabert | magali chabert
from the centre de neurochime du c.n.r.s., 5 rue blaise pascal, 67084 strasbourg cedex, france


دنیس weltin | denis weltin
from the centre de neurochime du c.n.r.s., 5 rue blaise pascal, 67084 strasbourg cedex, france


پاوول mandel | paul mandel
from the centre de neurochime du c.n.r.s., 5 rue blaise pascal, 67084 strasbourg cedex, france
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-298-695&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Pharmacology
نوع مقاله منتشر شده Original Research: Basic Science in Medicine
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