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Iranian Journal of Parasitology، جلد ۱۴، شماره ۳، صفحات ۳۷۹-۳۸۶
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عنوان انگلیسی Protein Detection of Excretory-Secretory Products and Somatic Extracts from Fasciola hepatica and F. gigantica Using Two-Dimensional Electrophoresis
چکیده انگلیسی مقاله Background: The aim of this research was to compare excretory-secretory and somatic extract materials of Fasciola hepatica and F. gigantica to detect protein maps of two species. Methods: Twenty infected livers were collected from sheep in industrial slaughterhouse in Tehran, 2017-2019. Worms were detached from bile ducts, then recognized according to morphologic and morphometric criteria. After three times washing, worms were incubated in RPMI culture media and excretory-secretory products were collected. Worms were crushed and homogenized for preparation of somatic extract. Two Dimensional Electrophoresis gels were accomplished for both excretory-secretory material and somatic extracts. Gels were scanned with densitometer and analyzed with Same Spots software and protein spots were identified with Expasy database. Results: For both excretory-secretory products and somatic extract, protein spots were appeared with two-dimensional electrophoresis technique. Quantitative analysis showed 40 and 28 protein spots for excretory-secretory of F. hepatica and F. gigantica respectively. For somatic extract 19 and 12 protein spots were recognized for F. hepatica and F. gigantica in that order. Conclusion: The rate of expression of some proteins were more in F. hepatica while expression of other proteins was high in F. gigantica. The expression of protease enzyme was higher in F. gigantica than F. hepatica. These data could be considered for biochemical differentiation of Fasciola species and subsequently to design and prepare of antigens for diagnosis/vaccine development.
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نویسندگان مقاله | Afshin RASOULI
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Ali FARAHNAK
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Hakimeh ZALI
Proteomics Research Center, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sci-ences, Tehran, Iran


| Mostafa REZAEIAN
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Abolfazl GOLESTANI
Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Mohammad Bagher MOLAEI RAD
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/2108
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-1924684.pdf
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