| چکیده انگلیسی مقاله |
Background: Pentavalent antimonials such as meglumine antimoniate (MA, Glucantime), are the first-line treatment against leishmaniasis, but at present, they have basically lost their efficacy. This study was aimed to explore epigallocatechin 3-O-gallate (EGCG), alone or in combination with MA against Leishmania tropica stages.
Methods: All experiments were carried out in triplicate using colorimetric assay, macrophage model, flow cytometry and quantitative real-time PCR. This experimental study was carried out in 2017 in Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Results: Promastigotes and amastigotes were more susceptible to EGCG than MA alone, but the effect was more profound when used in combination. EGCG exhibited high antioxidant level with a remarkable potential to induce apoptosis. Furthermore, the results showed that the level of gene expression pertaining to Th-1 was significantly up-regulated (P< 0.001).
Conclusion: EGCG demonstrated a potent anti-leishmanial effect alone and more enhanced lethal activity in combination. The principal mode of action entails the stimulation of a synergistic response and up-regulation of the immunomodulatory role towards Th-1 response against L. tropica. |
| نویسندگان مقاله |
| Morteza SADUQI
Department of Medical Parasitology and Mycology, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
| Iraj SHARIFI
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
| Zahra BABAEI
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
| Alireza KEYHANI
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
| Mahshid MOSTAFAVI
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
| Maryam HAKIMI PARIZI
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
| Mahdiyeh GHASEMIAN
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
| Mehdi BAMOROVAT
| Fatemeh SHARIFI
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
| Mohammad Reza AFLATOONIAN
Research Center of Tropical and Infectious Diseases, Kerman University of Medical Sciences, Kerman, Iran
| Fariba SHARIFIFAR
Herbal and Traditional Medicines Research Center, Department of Pharmacognosy, Kerman University of Medical Sciences, Kerman, Iran
| Pooya GHASEMI NEJAD
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
| Ahmad KHOSRAVI
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
| Ehsan SALARKIA
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran
| Rajender S. VARMA
Regional Center of Advanced Technologies and Materials, Faculty of Science, Palacký University in Olomouc, Šlechtitelů 27, 783 71 Olomouc, Czech Republic
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