| چکیده انگلیسی مقاله |
Background: Dirofilariasis is a globally distributed arthropod-borne parasitic disease of mainly canids and felids. We evaluated to extend the knowledge of morpho-molecular characteristics and outer ultrastructure of Dirofilaria immitis isolated from Northwest of Iran.
Methods: Overall, 67 filarial worms including 41 females and 26 males parasites were collected from the cardiovascular system of the 43 stray dogs in Meshkinshar, Ardebil Province, Northwest of Iran in 2017, and subjected to light and scanning electron microscopy (SEM) as well as carmine alum staining for morpho-molecular and identification. Molecular methods were used for confirmation of morphological findings by sequencing of Cytochrome c oxidase subunit I (cox1) gene.
Results: The partial DNA sequencing of cox1 gene of adult parasites showed considerable homology and close proximity to the previously isolated from Kerman and Meshkinshahr, Iran. The lowest genetic variation and the highest intra-species variability was found in D. immitis and Dirofilaria repens, respectively. No similarity was identified between D. immitis nucleotide sequence and Wolbachia species as its endosymbiont bacteria.
Conclusion: The SEM technique is an excellent tool for differential recognition of the parasite surface morphology and molecular techniques could differentiate and identify Dirofilaria spp. |
| نویسندگان مقاله |
| Majid KHANMOHAMMADI
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran .Department of Laboratory Sciences, Marand Branch, Islamic Azad University, Marand, Iran
| Lame AKHLAGHI
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
| Elham RAZMJOU
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
| Reza FALAK
Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran .Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
| Reza ZOLFAGHARI EMAMEH
Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
| Kobra MOKHTARIAN
Medical Plant Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
| Mehdi ARSHADI
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran .Al-Zahra Hospitals', Tabriz University of Medical Sciences, Tabriz, Iran
| Minoo TASBIHI
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
| Ahmad Reza MEAMAR
Department of Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
|