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Medical Journal of Islamic Republic of Iran، جلد ۳۵، شماره ۱، صفحات ۱۱۰۴-۱۱۱۲
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عنوان انگلیسی Upregulation of Ganglioside GD2 Synthase (GD2S), as a New Putative Cancer Stem Cell Marker in Breast Carcinomas
چکیده انگلیسی مقاله Background: GD2 synthase (GD2S) is the key enzyme required for ganglioside GD2 synthesis. It is commonly expressed in normal tissues and various cancers. Ganglioside GD2 is identified as a breast cancer stem cells (BCSCs) marker that promotes tumorigenesis. As GD2S has been found to be a useful molecular marker in neuroblastoma and retinoblastoma tumors, we suggest that it can be considered as a suitable candidate for the detection of CSCs in breast cancer tissues.     Methods: Expression of GD2S was examined in 65 breast tumors compared to adjacent normal tissues, applying quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA). The association between GD2S expression level and patients' clinical characteristics was also assessed.    Results: Our findings showed that GD2S mRNA expression was significantly higher in breast cancer tissues in comparison to normal adjacent tissue samples (4.92-fold change, p< 0.001) in advanced grades (p< 0.001) and stages (p< 0.001). It was also shown that GD2S protein expression was significantly higher in breast cancer tissues in comparison to normal adjacent tissues (4.86-fold change, p=0.010) in advanced grades (p=0.010), stages (p=0.005) and larger tumor size (p=0.002).    Conclusion: The current study showed that increased expression of GD2S in advanced breast cancer potentiates it as a promising tumor marker in these patients.
کلیدواژه‌های انگلیسی مقاله Breast cancer, Cancer Stem Cells, ELISA, GD2 Synthase, qRT-PCR
نویسندگان مقاله | Maryam Mansoori
Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, & Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran


| Alireza Mirzaei
Bone and Joint Reconstruction Research Center, Shafa Orthopedic Hospital, Iran University of Medical Sciences, Tehran, Iran


| Isa Abdi Rad
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran


| Rahim Mahmodlou
Department of Surgery, Imam Khomeini Hospital, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran


| Fatemeh Mansouri
Department of Genetics and Immunology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran


| Leili Saeednejad Zanjani
Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran


م | Zeynab Asadi- Lari
Department of Biology, Faculty of Science, University of Toronto, Toronto, Canada


| Zahra Madjd
Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, & Oncopathology Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-648-3&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cellular & Molecular Research
نوع مقاله منتشر شده Original Research
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