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Medical Journal of Islamic Republic of Iran، جلد ۳۵، شماره ۱، صفحات ۱۰۲۸-۱۰۳۱
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چکیده فارسی مقاله
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عنوان انگلیسی Correlation of serum microRNA-122 level with the levels of Alanine aminotransferase and HBV-DNA in Chronic HBV-infected patients
چکیده انگلیسی مقاله Background: The microRNA‐122 (miR‐122) is a liver‐specific microRNA that can be used as a potential molecular marker for predicting liver injury. There is a positive correlation between miR‐122 level in serum and hepatitis B virus (HBV) replication in patients infected with this virus. The present study was conducted to study the clinical importance and expression of miR-122 in asymptomatic and symptomatic patients with HBV infection in comparison to the healthy group.    Methods: This study was performed on 60 samples to examine the presence of HBsAg and total HBc antibody (IgM-IgG) using an enzyme-linked immunosorbent assay. HBV-DNA extraction and real time polymerase chain reaction (PCR) assay were performed on all samples via the Real ART HBV LC PCR kit on a LightCycler instrument. RNA was extracted from the serum of all participants. Next, miRNA expression was assessed using quantitative real time reverse-transcription PCR. Also, ALT levels were measured as a surrogate parameter for liver injury using Pars Azmoon Biochemical assay Kit on Hitachi autoanalyzer. The Levene , Kruskal Wallis, Mann-Whitney and Spearman's correlation tests were used for assessing the differences between the studied groups.    Results:  Based on the obtained results, miR-122 expression in patients with HBV without clinical symptoms was 1.6 times, while in patients with clinical symptoms was 2.7 times more than the control group (p=0.001). A significant increase was observed in the ALT enzyme of symptomatic patients (p=0.001). HBV DNA in the people with clinical symptoms was higher than 105 copies/mL and in the asymptomatic group was less than 103 copies/mL, suggesting a statistically significant increase in a group with clinical symptoms (p=0.001). Finally, it was found that the miR‐122 serum concentration correlated with HBV DNA and serum ALT (p=0.001).    Conclusion: Based on the obtained results, measuring the miR-122 levels can serve as a biomarker and an indicator of hepatitis B replication, especially in cases where ALT levels are unchanged; however, more research and more samples are needed.  
کلیدواژه‌های انگلیسی مقاله MicroRNA, Hepatitis B, MiR-122, ALT
نویسندگان مقاله | Rana Tabar Asad Laleh
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


| Zohreh Sharifi
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


| Ali Akbar Pourfathollah
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, & Departments of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-1473-3&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Virology
نوع مقاله منتشر شده Original Research
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