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Medical Journal of Islamic Republic of Iran، جلد ۳۵، شماره ۱، صفحات ۹۶۲-۹۶۸
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چکیده فارسی مقاله
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عنوان انگلیسی Human umbilical cord mesenchymal stem cells express cholinergic neuron markers during co-culture with amniotic membrane cells and retinoic acid induction
چکیده انگلیسی مقاله Background: A wide variety of cytokines are released from human amniotic membrane cells (hAMCs), which can increase the rate of differentiation of mesenchymal stem cells into the neurons. We studied the effect of Retinoic Acid (RA) on the differentiation rate of human Umbilical Cord Mesenchymal Stem Cells (hUMSCs) which were co-cultured with hAMCs.    Methods: In this experimental study, both hUMSCs and hAMCs were isolated from postpartum human umbilical cords and placenta respectively. The expression of mesenchymal (CD73, CD90 and CD105), hematopoietic and endothelial (CD34 and CD45) markers in hUMSCs were confirmed by flow cytometry. The hUMSCs were cultured in four distinct groups: group 1) Control, group 2) Co-culture with hAMCs, group 3) RA treatment and group 4) Co-culture with hAMCs treated by RA. Twelve days after culturing, the expression of NSE, MAP2 and ChAT differentiation genes and their related proteins were examined by real-time PCR and immunocytochemistry respectively.    Results: The flow-cytometry analysis indicated increased expression of mesenchymal markers and a low expression of both hematopoietic and endothelial markers (CD73:98.24%, CD90: 97.32%, CD105: 90.75%, CD34: 2.96%, and CD45:1.74%). Moreover, the expression of both NSE and MAP2 markers was increased significantly in all studied groups in comparison to the control group  On the other hand, the expression of ChAT had a significant increase in the group 2 and 4 (RA and RA+ co-culture).    Conclusion: RA can be used as an effective inducer to differentiate hUMSCs into cholinergic-like cells, and hAMCs could increase the number of differentiated cells as an effective factor.
کلیدواژه‌های انگلیسی مقاله Amniotic Membrane, Cell Differentiation, Mesenchymal Stem Cell, Neurons, Retinoic acid
نویسندگان مقاله | Mohammad-Reza Kouchakian
Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Morteza Koruji
Department of Anatomy, Faculty of Medicine, & Stem Cell and Regenerative Medicine Research Center, Iran University of Medical Scienc-es, Tehran, Iran


| Mohammad Najafi
Department of Biochemistry, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran


| Seyedeh Farzaneh Moniri
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Alireza Asgari
School of Pharmacy, Zanjan University of medical sciences, Zanjan, Iran


| Marjan Shariatpanahi
6. Department of Toxicology & Pharmacology, School of Pharmacy, International Campus, Iran University of Medical Sciences, Tehran, Iran


| Seyed Akbar Moosavi


| Hamid Reza Asgari
Department of Anatomy, Faculty of Medicine, & Stem Cell and Regenerative Medicine Research Center, & Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://mjiri.iums.ac.ir/browse.php?a_code=A-10-5928-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Cellular & Molecular Research
نوع مقاله منتشر شده Original Research
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