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Iranian Journal of Parasitology، جلد ۱۶، شماره ۳، صفحات ۳۶۶-۳۷۶
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عنوان انگلیسی Protective Effect of an Anti-HMGB-1 Neutralizing Antibody on Hemozoin-Induced Alveolar Epithelial Cell in a Model of Malaria Associated ALI/ARDS
چکیده انگلیسی مقاله Background: We aimed to determine whether neutralizing high mobility group box-1 (HMGB-1) prevents the release of HMGB-1 and proinflammatory cytokines on hemozoin (Hz)-induced alveolar epithelial cell in a model of malaria associated ALI/ARDS. Methods: This study was conducted in the Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand in 2020. Human pulmonary alveolar epithelial cells (HPAEpiCs) were exposed to medium alone or 20 µM Hz for 24 h and incubated with different concentrations (1, 5, and 10 µg/ml) of anti-HMGB-1 monoclonal antibody (mAb) for various times (0, 4, 12, 24, and 48 h). The levels of HMGB-1, TNF-α and IFN-γ in the supernatants were measured by ELISA. The mRNA expression of RAGE, TLR-2 and TLR-4 were analyzed by real-time PCR. Results: The HPAEpiCs treated with 10 µg/ml anti-HMGB-1 mAb showed a significant reduction in HMGB-1 release into the supernatant compared with those treated with 1 and 5 µg/ml anti-HMGB-1 mAb. The levels of TNF-α and IFN-γ were significantly decreased in the supernatant of HPAEpiCs treated with 1, 5, and 10 µg/ml anti-HMGB-1 mAb for 4, 12, 24, and 48 h compared with those stimulated with Hz alone. The mRNA expression levels of RAGE, TLR-2, and TLR-4 were significantly decreased after 24 h of anti-HMGB-1 antibody treatment at all concentrations. Conclusions: An anti-HMGB-1 antibody could be an effective agent for inhibiting the release of HMGB-1, TNF-α and IFN-γ. Furthermore, a neutralizing anti-HMGB-1 antibody could be applicable for the treatment of malaria-associated ALI/ARDS.
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نویسندگان مقاله | Tachpon Techarang
1. Department of Medical Sciences, School of Medicine, Walailak University, Nakhon Si Thammarat, Thailand 2. Tropical Medicine Research Unit, Research Institute for Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand


| Pitchanee Jariyapong
Department of Medical Sciences, School of Medicine, Walailak University, Nakhon Si Thammarat, Thailand


| Parnpen Viriyavejakul
Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand


| Supattra Glaharn
Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand


| Charit Srisook
Department of Tropical Pathology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand


| Chuchard Punsawad
1. Department of Medical Sciences, School of Medicine, Walailak University, Nakhon Si Thammarat, Thailand 2. Tropical Medicine Research Unit, Research Institute for Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3282
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