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Iranian Journal of Parasitology، جلد ۱۶، شماره ۳، صفحات ۴۰۲-۴۱۰
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عنوان انگلیسی An Enzyme-Linked Immunosorbent Assay for the Detection of Antibodies to Linguatula serrata in Experimentally Infected Dogs
چکیده انگلیسی مقاله Background: Linguatula serrata is a causative agent of visceral and nasopharyngeal linguatulosis in humans and animals. The aim of the present study was to investigate the immune response of dogs experimentally infected by L. serrata with ELISA. Methods: Five puppies were infected by inserting the L. serrata nymphs in their nasal cavities (infected group) in the Department of Parasitology of Shahid Chamran University of Ahvaz, during 2018-2019. Three animals were kept as the non-infected control group. Blood samples were collected from the animals for seven months at approximately monthly intervals for serum preparation. Nasal samples were taken weekly from the fourth month. ELISA was designed and performed on 64 sera (24 negatives, and 40 positives) using somatic (S), and excretory-secretory (ES) antigens. Results: Overall, 100% of the animals were infected with the parasite. Based on the results of ELISA, the ES antigen (sensitivity 95% and specificity 92%) was more preferred than the S antigen (sensitivity 95% and specificity 85%). Female parasites had significant effects on the immune response. There was a significant correlation between the clinical symptoms and the presence of female parasites (P< 0.05). Conclusion: The results showed a practical method for dogs' experimental infection. ELISA method is suitable for the detection of infection at different stages of development, especially before the maturation stage of the parasite. In this regard, the ES antigen of the parasite was more immunogenic. Therefore, ELISA can be used as a serological method in the early detection and epidemiological studies of infection with L. serrata in dogs.
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نویسندگان مقاله | Alireza Alborzi
Department of Parasitology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran


| Javad Pirkoreshk
Department of Parasitology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran


| Somayeh Bahrami
Department of Parasitology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran


| Masoud Ghorbanpoor
Department of Immunology, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran


| Mohammad Reza Tabandeh
Department of Biochemistry, School of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2858
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