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Iranian Journal of Parasitology، جلد ۱۶، شماره ۲، صفحات ۲۹۵-۳۰۴
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عنوان انگلیسی Detection and Molecular Characterization of Potentially Pathogenic Free-Living Amoebae from Recreational and Public Soils in Mazandaran, Northern Iran
چکیده انگلیسی مقاله Background: Free-living amoeba (FLA) belonging to Acanthamoeba spp., Naegleria, and Balamuthia mandrillaris are the soil-born protozoa. This study aimed to survey the occurrence of FLA, including Acanthamoeba spp., B. mandrillaris, Vermamoeba spp., and Naegleria spp., in soil samples collected from various districts of Mazandaran Province (Northern Iran) from July to December 2018. Methods: Overall, 118 soil samples from the recreational and public places were surveyed for the existence of Acanthamoeba spp., Vermamoeba, Naegleria, and B. mandrillaris using both morphological key and molecular tools with genus-specific primers of JDP1, NA, ITS1, and Bal, respectively. To verify the taxonomic status of isolated amoeba, the phylogenetic tree was made based on sequences of 18S rRNA by MEGA (5.05) software with the maximum likelihood model. Results: Overall, 61/118 samples (51.6%) were contaminated with FLA, and based on the sequencing data, 29 isolates were successfully sequenced. Among the samples, all isolated Acanthamoeba (52.4%) belonged to the T4 genotype with amplification of the DF3 region (18S rRNA gene). Internal transcribed spacer (ITS) sequencing revealed the presence of one strain of Naegleria americana. Twenty-eight V. vermiformis were also confirmed based on Nuclear SSU rDNA. Morphological survey and PCR assay did not show any positive samples for B. mandrillaris. Conclusion: The present study indicates the occurrence of FLA in soil sources of the recreational and public places in Mazandaran province that it can be a severe risk to human health. Thus, more studies are expected to survey the infection source in patients with FLA-related diseases.
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نویسندگان مقاله | Asal Tanzifi
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran


| Yousof Dadimoghadam
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran


| Samira Dodangeh
Department of Medical Parasitology and Mycology, Medical Microbiology Research Center, Qazvin University of Medical Scienc-es, Qazvin, Iran


| Ahmad Daryani
Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran


| Shahabeddin Sarvi
Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran


| Shirzad Gholami
Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran


| Seyed Abdollah Hosseini
Toxoplasmosis Research Center, Communicable Diseases Institute, Mazandaran University of Medical Sciences, Sari, Iran


| Tooran Nayeri Chegeni
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran


| Zahra Hosseininejad
Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2875
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