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Iranian Journal of Parasitology، جلد ۱۶، شماره ۱، صفحات ۱۴۶-۱۵۰
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عنوان انگلیسی Molecular and Serological Evaluation of Neospora caninum Infec-tion in Dogs from a Rural Setting in Fars Province, Southern Iran
چکیده انگلیسی مقاله Background: Dogs, as the definitive host of Neospora caninum, are important in the epidemiology of this parasitic infection. We aimed to determine the prevalence of N. caninum infection in a dog population from a rural setting in Fars Province, Southern Iran, using a combination of molecular and serological techniques. Methods: This cross-sectional study was carried out in Nov 2018 in three rural districts, Sar Mashhad, HosseinAbad, and Tolesaman located in Kazeroun Township in Fars province, southern Iran. Blood samples were taken from 60 stray and household dogs. Dogs’ sera were tested for antibodies against N. caninum, using a Neospora-Modified Agglutination Test. Moreover, dogs’ buffy coats were tested for Neospora DNA, using a molecular method. Results: Anti-Neospora antibodies were detected in sera of 4 out of 60 dogs, corresponding to a seroprevalence rate of 6.7%. Out of 25 female dogs, 1 was seropositive and of 35 males, 3 were seropositive, yet the differences were not statistically significant.  The infection was more prevalent in adult dogs (> 12 months), nevertheless, the differences between age and Neospora seropositivity was not statistically significant.  N. caninum DNA was not detected in the buffy coat of any of the studied dogs.  Conclusion: Findings of the study indicate that N. caninum is a common infection in dogs in rural areas of Fars province in southern Iran. The infected dogs might be a potentially important source of N. caninum infection to livestock in the area.
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نویسندگان مقاله | Morvarid HARIRI
1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Nasir AREFKHAH
2. Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran


| Fariba GHORBANI
1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Mehdi NAMAVARI
3. Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organi-zation (AREEO), Shiraz, Iran


| Mostafa OMIDIAN
1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Bahador SARKARI
1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran. 4. Basic Sciences in Infectious Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2795
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