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Iranian Journal of Parasitology، جلد ۱۶، شماره ۱، صفحات ۴۳-۵۱
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عنوان انگلیسی Activities of Andrographis paniculata (AS201-01) Tablet on Cox-2 and Prostaglandin Expression of Placental of Plasmodium berghei Infected Mice
چکیده انگلیسی مقاله Background: Placental malaria has ability to upregulate prostaglandin synthesis by increasing cyclooxygenase-2 (Cox-2) enzyme activity. Cox-2 and prostaglandin have a role in causing uterine contraction and therefore can cause abortion or preterm labor. Tablet AS201-01 containing the ethyl acetate fraction of Andrographis paniculata was tested in vivo on pregnant mice infected with Plasmodium berghei. AS201-01 inhibited the growth of P. berghei, increased TGF-β expression, decreased TLR-4 expression and apoptosis index of placental tissue in P. berghei infected pregnant mice and thus prevented placental malaria complications. These effects were correlated with the decrease of Cox-2 and prostaglandin expression. Methods: Twenty-four pregnant mice (Balb/c) were divided into 4 groups (n=6). Mice were maintained at Animal Laboratory of Institute of Tropical Disease, Universitas Airlangga, Surabaya, Indonesia in 2016. G1 were uninfected pregnant mice, G2 untreated infected pregnant mice, G3 infected pregnant mice treated with AS201-01, and G4 infected pregnant mice treated with DHP tablet. All infection groups (G2-G4) were inoculated with 1x106 of P. berghei parasite on day 9 of gestation and treated on day 11. All mice were terminated at day 15 of gestation, and placental tissue was collected. Cytokine expression of Cox-2 and prostaglandin were evaluated using immunohistochemistry. Results: G3 was found to have lower Cox-2 and prostaglandin expression compared to G4 and G2, but higher compared to G1. Cox-2 and prostaglandin expression was significantly different among groups (P< 0.001). Conclusion: This study demonstrates the ability AS201-01 tablets have to decrease Cox-2 and prostaglandin expression on placental of P. berghei infected mice and therefore eliminates the adverse effects of placental malaria. Methods: The influence of AS201-01 tablets on P. berghei infection in pregnant mice (G3) was tested by comparison with healthy (G1), untreated infected (G2) and infected mice treated with DHP (dihydroartemisinin-piperaquine phosphate) tablets (G4), a common treatment for malaria. All infection groups were inoculated with 1x106 of P. berghei parasite on day 9 of gestation and treated on day 11. All mice were terminated at day 15 of gestation, and placental tissue was collected. Cytokine expression of Cox-2 and prostaglandin were evaluated using immunohistochemistry. Results: G3 was found to have lower Cox-2 and prostaglandin expression compared to G4 and G2, but higher compared to G1. Cox-2 and prostaglandin expression was significantly different among groups (p< 0.001). Conclusions: This study demonstrates the ability AS201-01 tablets have to decrease Cox-2 and prostaglandin expression on placental of P. berghei infected mice and therefore eliminates the adverse effects of placental malaria.
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نویسندگان مقاله | Budi PRASETYO
1. Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 2. Soetomo General Academic Hospital, Surabaya, Indonesia


| Eka INDRIANI
1. Department of Obstetrics and Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia 2. Soetomo General Academic Hospital, Surabaya, Indonesia


| Nurya VIANDIKA
3. Graduate Student of Program Study Master of Health Reproduction, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia


| Hilkatul ILMI
4. Center of Natural Product Medicine Research and Development, Institute of Tropical Disease, Universitas Airlangga, Sura-baya, Indonesia


| Lidya TUMEWU
4. Center of Natural Product Medicine Research and Development, Institute of Tropical Disease, Universitas Airlangga, Sura-baya, Indonesia


| Aty WIDYAWARUYANT
4. Center of Natural Product Medicine Research and Development, Institute of Tropical Disease, Universitas Airlangga, Sura-baya, Indonesia 5. Department of Pharmaceutical Science, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2540
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