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Iranian Journal of Parasitology، جلد ۱۶، شماره ۱، صفحات ۱۱۱-۱۲۱
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عنوان انگلیسی Diagnosis of Acanthamoeba keratitis in Mashhad, Northeastern Iran: A Gene-Based PCR Assay
چکیده انگلیسی مقاله Background: The genus Acanthamoeba is a free-living opportunistic protozoan parasite, which widely distributed in soil and fresh water. Acanthamoeba keratitis, which causes a sight-threating infection of the cornea, is going to rise in Iran and worldwide. The aim of this study was to compare direct microscopy, culture and PCR for detection of Acanthamoeba spp. in clinical samples and to determine the genotypes of Acanthamoeba spp. by sequencing 18SrRNA gene. Methods: Among patients clinically suspected to AK referred to a tertiary ophthalmology center at Mashhad, northeastern Iran. During 2017-18, twenty corneal scrapes specimens obtained. The samples were divided into three parts, subjected to direct microscopic examination, culture onto non-nutrient agar and PCR technique.  Sensitivity, specificity, accuracy and likelihood ratio were evaluated. Results: Among 20 persons clinically suspected to amoebic keratitis, 13(69.2%) patients definitely diagnosed as Acanthamoeba keratitis. Wearing contact lens, eye trauma due to foreign particle and swimming in fresh water were the main predisposing factors. Most of patients suffered from pain and photophobia. Corneal ring infiltration and epithelial defect were common clinical sings. Direct examination had the lowest sensitivity and sensitivity of both Nelson-PCR and JDP-PCR methods were equal and highest. In addition, the results of sequencing identified that all strains belonged to T4 genotype. Conclusion: Amoebic keratitis is a sporadic parasitic keratitis, which is mainly seen in contact lens user in Mashhad. PCR based on 18S ribosomal DNA with JDP primers is a reliable and highly sensitive method for diagnosis of Acanthamoeba keratitis in clinically suspected cases.
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نویسندگان مقاله | Nazgol KHOSRAVINIA
1. Department of Medical Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Abdolmajid FATA
Department of Medical Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2. Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


| elham MOGHADDAS
2. Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


| Bibi Razieh HOSSEINI FARASH
1. Department of Medical Parasitology and Mycology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran 2. Cutaneous Leishmaniasis Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


| Mohammad Reza SADAGHAT
3. Khatam-al-Anbia Haspital, Mashhad University of Medical Sciences, Mashhad, Iran


| Ali Raza ESLAMPOUR
Khatam-al-Anbia Haspital, Mashhad University of Medical Sciences, Mashhad, Iran


| Lida JARAHI
4. Community Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2476
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