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Iranian Journal of Parasitology، جلد ۱۵، شماره ۴، صفحات ۵۱۱-۵۲۰
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عنوان انگلیسی Trypanothione Reductase Gene Mutations in Meglumine Antimoniate Resistant Isolates from Cutaneous Leishmaniasis Patients Using Molecular Dynamics Method
چکیده انگلیسی مقاله Background: In this study, mutations in the tripanothion reductase of Leishmania tropica isolated from Iran was investigated using sequencing and simulation of the enzyme by the molecular dynamic method. Methods: Fifteen susceptible and 15 clinical resistant L. tropica specimens were collected from skin lesions from different regions of Iran in 2017. After DNA extraction, trypanothione reductase (TRYR or TPR), gene fragment was amplified using PCR and sequencing methods. In the case of structural mutations, the components were simulated by molecular dynamics using the GROMACS software. Results: Some structural mutations were observed in 9 amino acids surrounding the active site of the TRYR gene of L. tropica with three-dimensional trypanothione reductase alteration. Conclusion: Change in the active site of TRYR of L. tropica, could probably contribute to the development of resistant L. tropica to glucantime. Because of the likely occurrence of mutations in glucantime as well as the ease of development of L. tropica resistant populations, more samples are needed to demonstrate the relationship between mutations in this enzyme and clinical resistance to glucantime. On the other hand, it is recommended that enzymatic studies be performed to confirm the role of mutation in the function and expression of trypanothione reductase in glucantime resistant and susceptible populations.
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نویسندگان مقاله | Fatemeh FOZONGARI
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Abdolhossein DALIMI
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Seid Shahriar ARAB
Department of Biophysics, Faculty of Biology Sciences, Tarbiat Modares University, Tehran, Iran


| Mehrdad BEHMANESH
Department of Genetic, Faculty of Biology Sciences, Tarbiat Modares University, Tehran, Iran


| Anahita KHAMMARI
Department of Biophysics, Faculty of Biology Sciences, Tarbiat Modares University, Tehran, Iran
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3172
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