| چکیده انگلیسی مقاله |
Background: When Angiostrongylus cantonensis develops from the third and fourth stage, it needs to change its host from the middle host, snail to the final host, rat. However, the mechanism involved in this change remains unclear.
Methods: The transcriptome differences of the third and fourth stages of A. cantonensis were explored by next-generation Illumina Hiseq/Miseq sequencing in China, in 2018.
Results: Overall, 137 956 488 clean reads and 20 406 213 373 clean bases of the two stages larvae were produced. Based on the queries against the Gene Ontology (GO), NCBI non-redundant protein sequences (Nr), Swissprot, and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases, 14 204 differentially expressed genes (DEGs) were predicted. GO enrichment analysis revealed 5660 DEGs with the top s categories as followings: biological process (GO:0008150, related to 5345 DEGs), cellular component (GO:0005575, related to 5297 DEGs), molecular function (GO:0003674, related to 5290 DEGs). In KEGG enrichment analysis, 116 genes were related to oxidative phosphorylation and 49 genes involved in the glycolytic process.
Conclusion: Metabolism changes, especially oxidative phosphorylation and glycolysis, might play a key role in A. cantonensis infection of its final rat host. Many other pathways might also contribute to the transcriptome changes between these two life stages. Overall, additional studies are needed for further details. |
| نویسندگان مقاله |
| Yue GUO
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China AND Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, Zhejiang, China
| Hong Chang ZHOU
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China AND Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, Zhejiang, China
| Ying DONG
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China
| Hai Yan DONG
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China AND Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, Zhejiang, China
| Yun Liang YAO
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China AND Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, Zhejiang, China
| Jing QIAN
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China AND Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, Zhejiang, China
| Hui ZHANG
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China
| Xiao Yu LI
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China
| Zhong Shan ZHANG
Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province, Huzhou University, Huzhou, Zhejiang, China
| Han Bing LIN
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China
| Tian ZHOU
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China
| Meng Jia ZHAO
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China
| Tang Qin JI
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China
| Run Ze WANG
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China
| Feng Ping ZHANG
School of Medicine, Huzhou University, Huzhou Cent Hosp, 759 Er Huan Rd, Huzhou, Zhejiang, China
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