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Iranian Journal of Parasitology، جلد ۱۵، شماره ۳، صفحات ۳۱۵-۳۲۰
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عنوان انگلیسی Comparison of in Vitro Efficacy of Six Disinfectants on the Hatching of Larval Eggs of Toxocara canis
چکیده انگلیسی مقاله Background: The environmental contamination with Toxocara canis eggs increases the risk of dissemination and transmission of the parasite in dogs and paratenic hosts such as humans. We aimed to evaluate different disinfectants to compare their effect on T. canis eggs. Methods: For its realization, 850 embryonated eggs were obtained, which were suspended in a solution of 5% formaldehyde and distilled water in Eppendorf tubes. In the tubes containing the 850 embryonated eggs, researchers was added 0.5 mL of each solution (enzymatic solution, sodium hypochlorite, iodopovidone, quaternary of ammonium, benzalkonium chloride, and super oxidation solution). After mixing, an aliquot was taken, observed under the microscope, and the number of broken eggs counted at different times to find the most effective ovicidal moment. Results: The enzymatic disinfectant present a significant difference (P = 0.05) with 276.06 broken eggs followed by ammonium with 105.20 broken eggs. After 10 min, the ammonium solution was the one that showed a significant difference of 50.50 hatched eggs, followed by the enzymatic 26.80 and hypochlorite 25.00 treatments. After 20 min, the enzymatic solution treatment showed a significant difference with the other solutions showing an increase of 98.80 broken eggs. In the 30 and 40-min times, only the enzymatic treatment showed a significant difference of 334.10 and 381.70 of broken eggs respectively. Conclusion: The enzymatic solution has the greatest ovicidal effect against the eggs of T. canis to present a greater number of broken eggs in a given time between 20 and 40 minutes.
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نویسندگان مقاله | Camilo ROMERO
Research Department, DERMAVET Veterinary Hospital, Mexico City, Mexico


| Rafael HEREDIA
Agricultural Sciences and Natural Resources, UAEM Amecameca University Center, Autonomous University of the State of Mexico, State of Mexico, Mexico


| Manuel BOLIO
Faculty of Veterinary Medicine and Zootechnics, Autonomous University of Yucatan, Mérida, Yucatán, México


| Laura MIRANDA
Research Department, DERMAVET Veterinary Hospital, Mexico City, Mexico


| Laura REYES
Research Department, DERMAVET Veterinary Hospital, Mexico City, Mexico


| Mauricio ARREDONDO
Department of Life Sciences, Guanajuato University, Irapuato-Salamanca Campus, Irapuato, Guanajuato, Mexico


| Ariadna FLORES
Agricultural Sciences and Natural Resources, UAEM Amecameca University Center, Autonomous University of the State of Mexico, State of Mexico, Mexico
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3057
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