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International Journal of Hematology-Oncology and Stem Cell Research، جلد ۱۵، شماره ۲، صفحات ۷۷-۸۹
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عنوان انگلیسی Decreased Inhibition of Proliferation and Induction of Apoptosis in Breast Cancer Cell Lines (T47D and MCF7) from Treatment with Conditioned Medium Derived from Hypoxia-Treated Wharton’s Jelly MSCs Compared with Normoxia-Treated MSCs
چکیده انگلیسی مقاله Background: Mesenchymal stem cells (MSCs) are an appealing source of adult stem cells for cell therapy due to the high rate of proliferation, self-renewal capability, and applicable therapy. Wharton’s jelly (WJ), the main component of the umbilical cord extracellular matrix, comprises multipotent stem cells with a high proliferation rate and self-renewal capability and has anti-cancer properties. MSCs have been reported to secrete a variety of cytokines that have a cytotoxic effect in various cancers. Oxygen tension affects MSCs proliferation, cytokines level but no in surface markers expression, MSCs’ differentiation. We explored the cytotoxic effect and inducing apoptosis of Wharton’s jelly derived mesenchymal stem cells (WJMSCs) secretions from normoxic WJMSCs (WJMSCs-norCM) (CM: conditioned medium) and hypoxic WJMSCs (WJMSCs-hypoCM) in breast cancer cell lines (T47D and MCF7). Materials and Methods: Cytotoxic activity was determined using the MTS assay. RT-PCR was performed to measure the expression of apoptosis-inducing genes, specifically P53, BAX, and CASP9, and the antiapoptotic gene BCL-2. Results: WJMSCs-norCM and WJMSCs-hypoCM were potent inhibitors of the proliferation in both cell lines. WJMSCs-norCM had more anticancer activity in T47D and MCF7. The IC50 value of WJMSCs-norCM on MCF7 was 42.34%, and on T47D was 42.36%. WJMSCs-norCM significantly induced the gene expression of apoptotic P53, BAX, and CASP9 and insignificantly decreased the antiapoptotic gene BCL-2 in both MCF7 and T47D cells. WJMSCs-CM has anticancer activity by inducing P53, BAX, and CASP9 apoptotic genes. Conclusion: WJMSCs-norCM has more anticancer activity than WJMSCs-hypoCM.
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نویسندگان مقاله | Wahyu Widowati
Faculty of Medicine, Maranatha Christian University, Jl. Prof. drg.. Suria Sumantri No.65, Bandung 40164, Indonesia


| Harry Murti
Stem Cell and Cancer Institute, Jl. A Yani No 2 Pulo Mas, Jakarta 13210, Indonesia


| Halida Widyastuti
Stem Cell and Cancer Institute, Jl. A Yani No 2 Pulo Mas, Jakarta 13210, Indonesia


| Dian Laksmitawati
Faculty of Pharmacy, Pancasila University, Jl. Raya Lenteng Agung No.56-80 Jakarta 12640, Indonesia


| Rizal Rizal
1) Biomolecular and Biomedical Research Center, Aretha Medika Utama,, Jl. Babakan Jeruk II No. 9, Bandung 40163, Indonesia 2)Biomedical Engineering, Department of Electrical Engineering, Faculty of Engineering, Universitas Indonesia, Jl. Kampus UI, Depok 16426, West Java, Indonesia


| Hanna Kusuma
Biomolecular and Biomedical Research Center, Aretha Medika Utama,, Jl. Babakan Jeruk II No. 9, Bandung 40163, Indonesia


| Sutiman Sumitro
Department of Biology, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Ketawanggede Malang 65145, Indonesia


| M Widodo
Pharmacology Laboratories, Faculty of Medicine, Brawijaya University Jl. Veteran, Ketawanggede Malang 65145,, Indonesia


| Indra Bachtiar
Stem Cell and Cancer Institute, Jl. A Yani No 2 Pulo Mas, Jakarta 13210, Indonesia
نشانی اینترنتی https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1539
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