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Medical Laboratory Journal، جلد ۱۶، شماره ۲، صفحات ۲۱-۲۶
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عنوان انگلیسی Nickel Nanoparticles/Recycled Polyethylene Terephthalate Nanofibers Reduce AlgD Expression in Pseudomonas aeruginosa
چکیده انگلیسی مقاله Background and objectives: Recycled polyethylene terephthalate (RPET) nanofibers have become an important part of human life, with a continuous increase in their production and consumption. Herein, the antibacterial activity of nickel nanoparticles/recycled polyethylene terephthalate nanofibers (NiNP/RPET NF web) was evaluated by analyzing alginate expression in Pseudomonas aeruginosa, as an opportunistic microorganism. Methods: NiNPs were synthesized and NiNP/RPET NF was produced by adding 25 μg/ml of NiNP to 10% solutions of RPET at a weight ratio of 3%. After exposing P. aeruginosa (PA01) to NiNP/RPET NF, the biofilm-forming capacity was determined and real-time PCR was performed to measure algD expression. Results: Treatment with 25 μg/ml of NiNP/RPET NF reduced growth of P. aeruginosa on Mueller Hinton agar but did not result in complete inhibition. The biofilm optical density (550 nm) was 0.464 ± 0.021 after treatment with NiNP/RPET NF and 0.082± 0.011 in the absence of NiNP/RPET NF. This indicates the significant reduction of biofilm formation after exposure to NiNP/RPET NF (p=0.01). In addition, a 0.6-fold (p=0.03) reduction in alginate expression was detected by real-time quantitative real-time PCR. Conclusion: Our results indicate the potential of NiNP/RPET NF for application in nano-based antibacterial medical systems.
کلیدواژه‌های انگلیسی مقاله Nickel nanoparticles, polyethylene terephthalate, recycling, Pseudomonas aeruginosa, Alginate
نویسندگان مقاله | Mohammad Hassan Jokar
Golestan Rheumatology Research Center, Sayad Shirazi Hospital, Golestan University of Medical Sciences, Gorgan, Iran


| Fatemeh Mohamadkhani
Department of Textile Engineering, Functional Fibrous Structures & Environmental Enhancement (FFSEE), Amirkabir University of Technology, Tehran, Iran


| Maliheh Moradzadeh
Golestan Rheumatology Research Center, Sayad Shirazi Hospital, Golestan University of Medical Sciences, Gorgan, Iran


| Samira Beygi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran


| Ashraf Mohamadkhani
Liver and Pancreatobiliary Diseases Research Center, Digestive Disease Research Institute, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://mlj.goums.ac.ir/browse.php?a_code=A-10-997-1&slc_lang=en&sid=1
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