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Medical Laboratory Journal، جلد ۱۵، شماره ۲، صفحات ۱-۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Expression Analysis of Circulating miR-21 in Iranian Patients with Hereditary Colorectal Cancer
چکیده انگلیسی مقاله Background and objectives: Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality in the world. MicroRNAs (miRNAs) have potential as diagnostic biomarkers for various diseases including cancer. This study was undertaken to investigate expression of miR-21 before and after surgery in patients with hereditary CRC. Methods: After collecting blood samples from 39 patients and 39 healthy controls, total RNA was extracted by the TRIzol method. Following cDNA synthesis, expression of miR-21 in serum of subjects was evaluated using real-time PCR, along with two reference genes, let-7d and let-7g. The real-time expression results and Ct values were collected and analyzed based on the 2-∆∆ct method. Results: In spite of tumor removal, serum miR-21 expression levels was significantly higher in hereditary CRC patients compared with controls (P=0.022). Conclusion: Our results confirmed that samples from hereditary cases of CRC must not be included in experiments on the diagnostic potential of miRNAs.
کلیدواژه‌های انگلیسی مقاله Colorectal Neoplasms,Gene Expression,Real-Time Polymerase Chain Reaction
نویسندگان مقاله | Mohammad Arefi
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran


| Abbas Abdollahi
Department of General Surgery, School of Medicine, Surgical Oncology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran


| Ayyoob Khosravi
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran


| Abdolavahab Moradi
Department of Microbiology, School of Medicine, Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran


| Seyed Hamid Aghaee-Bakhtiari
Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Naimeh Javid
Department of Microbiology, School of Medicine, Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran


| Mehdi Evazalipour
Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran


| Anvarsadat Kianmehr
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran
نشانی اینترنتی http://mlj.goums.ac.ir/browse.php?a_code=A-10-854-1&slc_lang=en&sid=1
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