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Molecular Biology Research Communications، جلد ۱۰، شماره ۳، صفحات ۱۰۵-۱۰۸
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عنوان انگلیسی Prokaryotic expression of chimeric GFP-hFc protein as a potential immune-based tool
چکیده انگلیسی مقاله GFP is an old-yet-powerful protein marker, which has been widely used in molecular biotechnology due to its capacity of exhibiting bright green fluorescence when exposed to ultraviolet light. The hFc region of IgG antibodies is a specific binding ligand of expressed receptors on immune cells with well-known cellular-associated functions like opsonization and phagocytosis. In this present study, we proceeded to fuse gfp-hfc gene into pET-28a to create a recombinant pET-28a-gfp-hfc vector. The expression of GPF-hFc was induced by IPTG and confirmed using SDS-PAGE and followed by Western blot probed with 6xHis antibodies. This chimeric protein was utilized in specific binding experiments with protein A/G-coated magnetic beads using a fluorescence microscope. Due to its fluorescence and binding ability, GFP-hFc could be used as a model molecule for monitoring molecule detection studies, tracking nanoparticle migration and distribution, or stimulating immune responses.
کلیدواژه‌های انگلیسی مقاله GFP, hFc-tag, GPF-hFc, protein A/G-coated magnetic beads
نویسندگان مقاله Thanh-Tan Nguyen |
Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam

Hai-Vy Vo-Nguyen |
Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam

Hieu Tran-Van |
Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, Ho Chi Minh City, Vietnam

نشانی اینترنتی https://mbrc.shirazu.ac.ir/article_6154_ad0b8cd1539f3ec975fd621061d41684.pdf
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