| چکیده انگلیسی مقاله |
Background: Urtica dioica (UD), as a natural antioxidant, has positive effects on oocyte maturation. This study aimed to investigate the effects of hydro-alcoholic UD extract and retinoic acid on follicular development in an in vitro fertilization (IVF) condition. Methods: A total of 40 female Wistar rats were randomly divided into 5 groups: group 1 received normal saline, group 2 was given 25 mg/kg retinoic acid, group 3 was administered with 100 mg/kg UD extract, group 4 was treated with retinoic acid plus UD extract, and group 5 received 10 mg/kg olive oil. The histomorphometric parameters were analyzed, including the number of follicles, follicular atrophy, fertilized oocytes, 2-cell embryos, dead embryos, and blastocysts. Results: Retinoic acid caused a significant increase in the primary, preantral, and atretic follicles and a substantial decrease in the corpus luteum compared with the control group (p< 0.001). The number of preantral, antral follicles, and corpus luteum was significantly higher in group 3 compared with group 1 (p< 0.001). Moreover, coadministration of UD plus retinoic acid (group 4) significantly reduced the atretic follicles (p< 0.05). Conclusion: Based on the results, UD herbal extract, as a natural antioxidant agent, could reduce the adverse effects of retinoic acid on oocyte maturation in an IVF condition. |
| نویسندگان مقاله |
| Saade Abdalkareem Jasim
Al-Maarif University College, Medical Laboratory Techniques Department, Al-anbar-Ramadi, Iraq
| Sana Abdul-Jabbar Ali
Pharmacy Department, Al-Safwa University College, Karbalaa, Iraq
| Osama Q. Fadhil
Pharmacy Department, Al-Safwa University College, Karbalaa, Iraq
| Muqaddas Kh. Rakhmatova
Department of Histology and Medical Biology, Tashkent State Dental Institute, Makhtumkuli Street 103, Tashkent, Uzbekistan
| Hamzah H. Kzar
Veterinary Medicine College, Al-Qasim Green University, Al-Qasim, Iraq
| Ria Margiana
Department of Anatomy, & Master's Programme Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, & Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
| Moaed E. Al-Gazally
College of Medicine, University of Al-Ameed, Karbala, Iraq
| Abduladheem Turki Jalil
Medical Laboratories Techniques Department, Al-Mustaqbal University College, Babylon, Hilla, 51001, Iraq
| Zaid Hameed Mahmood
Diyala University, College of Science, Department of Chemistry, Iraq
| Surendar Aravindhan
Department of Pharmacology, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences, Chennai, India
| Yasser Fakri Mustafa
Department of Pharmaceutical Chemistry, College of Pharmacy, University of Mosul, Mosul-41001, Iraq
| Mohammed Q. Sultan
College of Dentistry, Al-Ayen University, Thi-Qar, Iraq
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