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Iranian Journal of Microbiology، جلد ۸، شماره ۵، صفحات ۲۸۲-۲۸۷
عنوان فارسی
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کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Comparison of two methods fordetection of E. coli O157H7 in unpasteurized milk
چکیده انگلیسی مقاله Background and Objectives: The most common serotype of enterohaemorrhagic Esherichia coli group or Shiga-toxin-producing E. coli is O157:H7. Domestic and wild ruminants are regarded as the main natural reservoirs. O157:H7 serotype is the major cause of gastrointestinal infections in developed countries. In this study was conducted to survey on the toxigenic E. coli O157: H7 strains in milk of industrial dairy farms. Materials and Methods: A total number of 150 milk samples were collected from dairy industry in Khuzestan, over a period of 6 months and were evaluated by cultivation in selective media (CT-SMAC) and multiplex PCR. Results: Two isolates were identified as E. coli using biochemical tests, none of them were toxigenic E. coli O157:H7 as determined by multiplex PCR. Using direct PCR on milk samples, 45 samples contained at least one gene of the studied genes in this investigation ( rfb, flic, stx1 , stx2 ). With direct PCR, 2 milk samples were positive for toxigenic O157:H7. Conclusion: E. coli O157:H7 is present in this region and so the necessity for strict compliance of health standards is recommended. This is the first study on O157: H7 E. coli milk contamination in Khuzestan province. Based on these results, direct PCR is more accurate than indirect PCR.
کلیدواژه‌های انگلیسی مقاله Milk, Escherichia coli O157,H7, PCR
نویسندگان مقاله مریم فرهمندفر | maryam farahmandfar


نغمه موری بختیاری | naghmeh moori bakhtiari
department of microbiology, faculty of veterinary medicine, shahid chamran university of ahvaz, iran

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

سعد gooraninezhad | saad gooraninezhad
2department of clinical sciences, faculty of veterinary medicine, shahid chamran university of ahvaz, iran

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

مهدی زارعی | mehdi zarei
3department of food hygiene, faculty of veterinary medicine, shahid chamran university of ahvaz, iran

سازمان اصلی تایید شده: دانشگاه شهید چمران (Shahid chamran university)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/825
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/91/article-91-269900.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Articles
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