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Iranian Journal of Parasitology، جلد ۱۸، شماره ۳، صفحات ۳۵۱-۳۶۱
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عنوان انگلیسی New Primers for Detection and Differentiation between Leishmania viannia and L. leishmania Subgenera by Polymerase Chain Reaction
چکیده انگلیسی مقاله Background: Leishmania is the parasitic protozoan responsible for leishmaniases, a disease that can cause a range of cutaneous, mucosal, and visceral infections. Two subgenera L. Viannia and L. Leishmania are known to infect humans in the tropics and subtropics of the Americas. The aim of the present study was to develop a new pair of primers for the two subgenera and test in clinical samples. Methods: We designed two new pairs of primers for a PCR method from two conserved genes, cysteine proteinase B (cpb) and N-acetylglucosamine-6-phosfate deacetylase-like protein (nagA), as specific markers for those two respective subgenera. Primers were tested with 16 microscopical positive clinical samples from the Amazon region of Ecuador obtained in 2010-2020 period. Results: The cpb presented a band of 172 bp and the nagA a band of 300 bp, thus clearly differentiating L. viannia from L. leishmania. Additionally, primers identified and differentiated the clinical samples in the two subgenera. Conclusion: The new primers targeting different two genes and standardized in a PCR assay could identified and differentiated Leishmania parasites at subgenus level. This protocol could be used for Leishmania genus identification and diagnosis at the subgenus level and for determining the parasite's geographical distribution where different Leishmania subgenera are found in the same area.
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نویسندگان مقاله | Manuel Calvopina
One Health Research Group, Faculty of Medicine, Universidad de las Américas (UDLA), Quito, Ecuador


| David Fonseca-Carrera
Biotechnology Engineering, Faculty of Engineering and Applied Sciences (FICA), Universidad de las Américas (UDLA), Quito, Ecuador


| Irina Villacrés-Granda
Biotechnology Engineering, Faculty of Engineering and Applied Sciences (FICA), Universidad de las Américas (UDLA), Quito, Ecuador


| Alberto Toapanta
Biotechnology Engineering, Faculty of Engineering and Applied Sciences (FICA), Universidad de las Américas (UDLA), Quito, Ecuador


| Carlos Chiluisa-Guacho
National Institute for Investigation in Public Health “Leopoldo Izquieta Pérez (INSPI)”, Tena, Ecuador


| Carlos Bastidas-Caldes
Doctoral Program in Public and Animal Health, Universidad de Extremadura, Extremadura, Spain
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3513
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