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Journal of Medical Bacteriology، جلد ۱۲، شماره ۱، صفحات ۳۳-۴۲
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عنوان انگلیسی Comparative Evaluation of ELISA and Real-time PCR Tests to Detect COVID-19
چکیده انگلیسی مقاله Background:   Considering the wide spread of covid-19 and its high death rate, it is very important to find a sensitive and accurate diagnostic method. Thus, this study compared two main diagnostic approaches; PCR and ELISA, to detect COVID-19. Methods:   Fifty patients admitted to Baghiyatalah Hospital were examined to detect COVID-19 RNA by Real-time PCR method, as well as for the presence of IgG and IgM antibodies by ELISA method. The results were statistically analysed by SPSS software. Results:   The mean age of patients is 38.4 years old. The percentage of positive cases of COVID-19 in the studied patients according to PCR and ELISA tests was 66% and 70%, respectively. There was a statistically significant difference between positive cases of COVID-19 detected by PCR and ELISA with emerging fever, weakness, and lethargy . The diagnostic value of ELISA versus PCR showed that the sensitivity, specificity, positive likelihood ratio, and true positive rate were 100%, 88.2%, 8.5, and 94.29%, respectively. Conclusion:   Although the sensitivity of detection in Real-time PCR is higher than that in ELISA, there is a high agreement between the two methods when used for diagnosis of COVID-19.
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نویسندگان مقاله | Saye Moshashei
non


| Haniyeh Bashi Zadeh Fakhar
Department of Human Geneticse, Science And Research Branch, Branch, Islamic Azad University, Tehran, Iran


| Babak Shaghaghi
0000-0001-8225-786X


| Melika Jalalian
0000-0001-5001-0918
نشانی اینترنتی https://jmb.tums.ac.ir/index.php/jmb/article/view/503
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