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Iranian Journal of Basic Medical Sciences، جلد ۲۷، شماره ۵، صفحات ۵۸۸-۵۹۵
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عنوان انگلیسی Cadmium-induced annulus fibrosus cell senescence contributes to intervertebral disc degeneration via the JNK/p53 signaling pathway
چکیده انگلیسی مقاله Objective(s): Investigating the impact of cadmium (Cd) on annulus fibrosus (AF) cells and its potential mechanism was the purpose of the current study.Materials and Methods: Cd was cultivated in different concentrations (0, 1, 5, 10, and 20 μM) on AF cells and the potential effects of the metal were assessed. Using the CCK-8 method, cell viability and proliferation were identified. Using transcriptome analysis, the annulus fibrosus cells were sequenced both with and without cadmium chloride. The EdU method was used to determine the rate of cell proliferation; senescence-associated β-galactosidase (SA-β-Gal) staining was used to determine the number of positive cells; and western blot, RT-PCR, and immunofluorescence were used to determine the protein and mRNA expression of senescence-associated proteins (p16, p21, and p53) and c-Jun N-terminal kinase (JNK).Results: According to the findings, Cd has the ability to increase the production of senescence-associated genes (p16 and p21) and senescence-associated secreted phenotype (SASP), which includes IL-1β and IL-6. Through the JNK/p53 signal pathway, Cd exposure simultaneously accelerated AF cell senescence and promoted SASP. Following JNK inhibitor (SP600125) treatment, the expression of p53, JNK, and senescence-associated indices were all down-regulated.Conclusion: By activating the JNK/p53 signaling pathway, Cd can induce oxidative stress damage and AF cell senescence. These findings could provide a new approach for treating and preventing intervertebral disc degeneration (IVDD) caused by Cd exposure.
کلیدواژه‌های انگلیسی مقاله Annulus fibrosus, Cadmium, c-Jun N-terminal kinase, Intervertebral disc-degeneration, Senescence, Tumor suppressor protein- p53
نویسندگان مقاله | Xin Liu
Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu, China


| Wenjie Zhao
Department of Orthopedics, Dalian Medical University, Dalian 116000, Liaoning, China


| Man Hu
Department of Orthopedics, Dalian Medical University, Dalian 116000, Liaoning, China


| Yu Zhang
Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu, China


| Jingcheng Wang
Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu, China


| Liang Zhang
Department of Orthopedics, Clinical Medical College of Yangzhou University, Yangzhou 225001, Jiangsu, China
نشانی اینترنتی https://ijbms.mums.ac.ir/article_23689.html
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