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Dental Research Journal، جلد ۱۳، شماره ۶، صفحات ۰-۰
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عنوان انگلیسی Effects of alkaline treatment for fibroblastic adhesion on titanium
چکیده انگلیسی مقاله Background: The surface energy of titanium (Ti) implants is very important when determining hydrophilicity or hydrophobicity, which is vital in osseointegration. The purpose of this study was to determine how Ti plates with an alkaline treatment (NaOH) affect the adhesion and proliferation of human periodontal ligament fibroblasts (HPLF). Materials and Methods: In vitro experimental study was carried out. Type 1 commercially pure Ti plates were analyzed with atomic force microscopy to evaluate surface roughness. The plates were treated ultrasonically with NaOH at 5 M (pH 13.7) for 45 s. HPLF previously established from periodontal tissue was inoculated on the treated Ti plates. The adhered and proliferated viable cell numbers were determined using the 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide method for 60 min and 24 h, respectively. The data were analyzed using Kruskal–Wallis tests and multiple comparisons of the Mann–Whitney U‑test, P value was fixed at 0.05. Results: The mean roughness values equaled 0.04 μm with an almost flat surface and some grooves. The alkaline treatment of Ti plates caused significantly (P < 0.05) more pronounced HPLF adhesion and proliferation compared to untreated Ti plates. Conclusion: The treatment of Ti plates with NaOH enhances cell adhesion and the proliferation of HPLF cells. Clinically, the alkaline treatment of Ti‑based implants could be an option to improve and accelerate osseointegration. Key Words: Cells, periodontal ligament, sodium hydroxide, titanium, wettability
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نویسندگان مقاله miryam cuellar‑flores | miryam cuellar‑flores


لورا سوسانا acosta‑torres | laura susana acosta‑torres


عمر martínez‑alvarez | omar martínez‑alvarez


بنیامین sánchez‑trocino | benjamin sánchez‑trocino


خاویر de la fuente‑hernández | javier de la fuente‑hernández


rigoberto garcia‑garduño | rigoberto garcia‑garduño


rene garcia‑contreras | rene garcia‑contreras


نشانی اینترنتی http://drj.mui.ac.ir/index.php/drj/article/view/1839
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