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Avicenna Journal of Phytomedicine، جلد ۱۴، شماره ۳، صفحات ۳۴۹-۳۶۴
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عنوان انگلیسی Auraptene inhibits migration, invasion and metastatic behavior of human malignant glioblastoma cells: An in vitro and in silico study
چکیده انگلیسی مقاله Objective: The present work examined the anti-metastatic effects of auraptene and their underlying mechanisms of action in U87 Glioblastoma multiforme (GBM) cells.Materials and Methods: To test the hypothesis, cell culture, Matrigel invasion assay, scratch wound healing assay, gelatin zymography assay, qRT-PCR, and western blot analysis were conducted.Results: At sublethal concentrations of 12.5 and 25 µg/ml, auraptene exhibited a significant reduction in cell invasion and migration of U87 cells, as assessed using scratch wound healing and Transwell tests, respectively. The qRT-PCR and zymography experiments demonstrated a significant decrease in both mRNA expression and activities of MMP-2 and MMP-9 following auraptene treatment. Western blot analysis also showed that the total protein level of MMP-2 as well as phosphorylation of crucial metastasis-related proteins, including p-JNK and p-mTOR, decreased in auraptene-treated cells. The molecular docking studies consistently demonstrated that auraptene exhibits a significant affinity towards MMP-2/-9, the ATP binding site of mTOR and JNK1/2/3.Conclusion: Auraptene effectively inhibited the migration and invasion of GBM cells. This inhibitory effect was induced by modulating specific mechanisms, including suppressing MMPs, JNK, and mTOR activities. Auraptene might serve as a potential anti-metastatic agent against malignant GBM.
کلیدواژه‌های انگلیسی مقاله Auraptene, Glioblastoma multiforme, Migration, Invasion, Metastasis
نویسندگان مقاله | Seyed Hadi Mousavi
Medical Toxicology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran


| Mohammad Jalili-Nik
Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Mohammad Soukhtanloo
Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Arash Soltani
Department of Clinical Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran


| Farzaneh Abbasinezhad-Moud
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran


| Hamid Mollazadeh
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical ‎Sciences, Bojnurd, Iran


| Farzaneh Shakeri
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical ‎Sciences, Bojnurd, Iran


| Bahram Bibak
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical ‎Sciences, Bojnurd, Iran


| Amirhossein Sahebkar
Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran


| Amir R. Afshari
Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical ‎Sciences, Bojnurd, Iran
نشانی اینترنتی https://ajp.mums.ac.ir/article_23586.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Research Article
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