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Iranian Journal of Parasitology، جلد ۱۱، شماره ۴، صفحات ۴۸۰-۴۸۸
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی Modulation of the Immune Response to DNA Vaccine Encoding Gene of 8-kDa Subunit of Echinococcus granulosus Antigen B Using Murine Interleukin-12 Plasmid in BALB/c Mice
چکیده انگلیسی مقاله Background : The current study was designed to evaluate immune responses induced by DNA vaccines encoding 8-kDa subunit of antigen B (HydI) of Echinococcus granulosus and murine interleukin 12 (IL-12) as genetic adjuvants in BALB/c mice. Methods : Expression plasmid pcDNA3.1 containing HydI (pcHyd1) as vaccine along with the murine interleukin 12 (pcMIL12) as adjuvant were used. Thirty-five mice in the five experimental groups received PBS, empty pcDNA3.1, pcHydІ, pcMIL-12, and pcHydІ+ pcMIL-12 in days zero, 14 th and 28 th . Two weeks after the last immunization, evaluation of the immune response was performed by evaluating the proliferation of splenic lymphocytes, IFN-γ and IL-4, determination of IgG isotyping titer. Results: Mice that received the pcHydI+pcMIL12 exhibited higher levels of lymphocyte proliferation compared to mice that received the pcHydI alone ( P < 0.001), and produced significantly more IFN-γ in comparison to other groups ( P < 0.001). In addition, they produced significantly less IL-4 than mice receiving the PBS and the empty plasmid ( P < 0.023). The IgG2a levels were clearly higher in pcHydI+pcMIL12 group in comparison with the groups of pcHydI alone, empty plasmid, and PBS. In contrast, IgG1 was elevated in the group of pcHydI. Conclusion: Co-delivery of IL-12 with DNA encoding 8-kDa subunit of antigen B was effective significantly in inducing the immune response in mice.
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نویسندگان مقاله حکیم عزیزی | hakim azizi
dept. of medical parasitology, zabol university of medical sciences, zabol, iran dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

بهرام کاظمی | bahram kazemi
cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran dept. of medical biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مژگان بنده پور | mojgan bandehpour
dept. of medical biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

مهدی محبعلی | mehdi mohebali
dept. of medical parasitology, zabol university of medical sciences, zabol, iran center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

علی خامسی پور | ali khamesipour
skin and leprosy research center, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

مژگان aryaeipour | mojgan aryaeipour
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

هاجر یعقوبی | hajar yaghoobi
dept. of medical biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

محمد باقر رکنی | mohammad bagher rokni
dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1347
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-313549.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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