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Iranian Journal of Parasitology، جلد ۱۱، شماره ۴، صفحات ۴۸۹-۴۹۸
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عنوان انگلیسی Species Identification and Molecular Typing of Leishmania Spp. Using Targeting HSP70 Gene in Suspected Patients of Cutaneous Leishmaniasis from Sistan and Baluchestan Province, Southeast Iran
چکیده انگلیسی مقاله Background: Leishmaniasis is a sand fly-borne disease caused by the protozoan parasites belonging to the genus Leishmania . Because of the preventing and controlling methods, clinical course, prognosis and choice of treatment are differing from species; differentiation of species is critical. The present study was aimed to detect the parasite species using the PCR-RFLP method. Methods: A total of 130 Giemsa-Stained slides from suspected Cutaneous leishmaniasis (CL) patients were examined under a light microscope at×1000. DNA from each slide was extracted PCR method was undertaken with HSP70 genes and the PCR products were digested with a restriction enzyme HaeIII (BsuR1). The study was conducted in the laboratory of Zahedan University of Medical Sciences in the Sistan and Baluchestan Province, southeastern Iran in 2015. Results: From 130 suspected samples, 59 (45.3%) were positive by the microscopic examination, meanwhile 64 (49.2%) were positive by PCR-RFLP, Leishmania species were recognized, and L. tropica was introduced as predominant species in current study . Conclusion: PCR-RFLP is a valuable technique for distinguish of Leishmania species. Furthermore, anthroponotic CL is the dominant cause of CL in Sistan and Baluchestan Province.
کلیدواژه‌های انگلیسی مقاله Leishmania, HSP70 gene, PCR-RFLP, Human, Iran
نویسندگان مقاله هادی میراحمدی | hadi mirahmadi
infectious diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, iran dept. of parasitology, mycology and entomology, faculty of medicine, zahedan university of medical sciences, zahedan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی زاهدان (Zahedan university of medical sciences)

علیرضا سلیمی خراشاد | alireza salimi khorashad
infectious diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, iran dept. of parasitology, mycology and entomology, faculty of medicine, zahedan university of medical sciences, zahedan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی زاهدان (Zahedan university of medical sciences)

علیرضا sohrabnahad | alireza sohrabnahad
dept. of parasitology, mycology and entomology, faculty of medicine, zahedan university of medical sciences, zahedan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی زاهدان (Zahedan university of medical sciences)

پیمان حیدریان | peyman heydarian
dept. of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نگار بیژنی | negar bizhani
dept. of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1348
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-313550.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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