| چکیده انگلیسی مقاله |
Background: As a waterborne pathogen, Cryptosporidium is one of the most common causes of gastroenteritis in human and hoofed livestock animals. This study aimed to investigate the distribution of Cryptosporidium spp. in human and livestock wastewaters in Iran, by the 18S rRNA sequence analysis. Methods: A total of 54 raw wastewater samples collected from three urban treatment plants and two slaughterhouses during 2014-2015 in Tehran, Iran. The presence of the Cryptosporidium oocysts was assessed by immunofluorescence with monoclonal antibodies. To characterize the oocysts at the molecular level, the 18S rRNA gene of Cryptosporidium was PCR amplified and sequenced. Results: Of the 54 wastewater samples examined, 34 (62.9%) were positive for Cryptosporidium oocysts using the IFA. Of these, 70.5% (24/34) were positive by PCR, that 91.6% (22/24) were successfully sequenced. The species of C. andersoni (95.4%) and C. xiaoi (4.6%) were detected in livestock wastewater samples. Conclusion: C. andersoni was the major Cryptosporidium sp. found in the aquatic environmental wastewater samples. The high rate of detection of C. andersoni in domestic wastewater was probably the result of the predominancy of this species in cattle herds in Iran. The current study is the first report of C. xiaoi in Iran. |
| نویسندگان مقاله |
کریم حاتم نهاوندی | kareem hatam nahavandi
department of parasitology and mycology, school of medicine, zahedan university of medical sciences, zahedan, iran department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran infectious diseases and tropical medicine research center, zahedan university of medical sciences, zahedan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی زاهدان (Zahedan university of medical sciences)
مهدی محبعلی | mehdi mohebali
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
امیر حسین محوی | amir hossein mahvi
department of environmental health engineering, school of public health, tehran, iran center for water quality research, institute for environmental research ier , tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
حسین کشاورز | hossein keshavarz
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
حمید رضا نجفیان | hamid reza najafian
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
حامد میرجلالی | hamed mirjalali
foodborne and waterborne diseases research center, research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran gastroenterology and liver disease research center, research institute for gastroenterology and liver diseases, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
ساسان رضایی | sasan rezaei
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
مصطفی رضاییان | mostafa rezaeian
department of parasitology and mycology, school of public health, tehran university of medical sciences, tehran, iran center for research of endemic parasites of iran crepi , tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
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