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Iranian Journal of Parasitology، جلد ۱۱، شماره ۴، صفحات ۵۰۷-۵۱۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Molecular Characterization of Trichomonas vaginalis Strains Based on Identifying Their Probable Variations in Asymptomatic Patients
چکیده انگلیسی مقاله Background: The aim of this study was to identify the Trichomonas vaginalis strains/haplotypes based on identifying their probable variations in asymptomatic patients referred to Tabriz health centers, northwestern Iran. Methods: Sampling was taken from 50-suspected women to T. vaginalis in northwestern Iran. The obtained samples were smeared and cultured. Fifty DNA samples were extracted, amplified and identified by nested polymerase chain reaction and PCR-RFLP of actin gene using two endonuclease enzymes: M seI and R saI. To reconfirm, the amplicons of actin gene were directly sequenced in order to identify the strains/haplotypes. Results: PCR-RFLP patterns, sequencing and phylogenetic analyses revealed definitely the presence of the G (n=22; 73.4%) and E (n=8; 26.6%) strains. Multiple alignments findings of genotype G showed five haplotypes and two amino acid substitutions in codons 192 and 211 although, no remarkable unique haplotype was found in genotype E. Conclusion: The accurate identification of T. vaginalis strains based on discrimination of their unknown haplotypes particularly those which are impacted on protein translation should be considered in parasite status, drug resistance, mixed infection with HIV and monitoring of asymptomatic trichomoniasis in the region.
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نویسندگان مقاله عادل اسپوتین | adel spotin
immunology research center, tabriz university of medical sciences, tabriz, iran department of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

ساناز تقی زاده اقتدار | sanaz taghizadeh eghtedar
immunology research center, tabriz university of medical sciences, tabriz, iran department of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

عباس شهبازی | abbas shahbazi
immunology research center, tabriz university of medical sciences, tabriz, iran department of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

اصغر صالح پور | asghar salehpour
department of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

صدیقه سرافراز | seddigheh sarafraz
department of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran east azerbaijan province health center, tabriz health center, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

سید علی شریعت زاده | seyyed ali shariatzadeh
department of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

محمود محامی اسکویی | mahmoud mahami oskouei
immunology research center, tabriz university of medical sciences, tabriz, iran department of parasitology and mycology, faculty of medicine, tabriz university of medical sciences, tabriz, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تبریز (Tabriz university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1350
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-313552.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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