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Iranian Journal of Parasitology، جلد ۱۹، شماره ۲، صفحات ۱۵۳-۱۶۱
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عنوان انگلیسی Designing a Multiple-Epitope Vaccine Candidate against Leishmania major and Leishmania infantum for Monocyte-Derived Exosome Preparation
چکیده انگلیسی مقاله Background: Leishmania is a vector-borne protozoon, which causes visceral, cutaneous and mucocutaneous leishmaniosis in human and animals. Monocyte-derived exosome vaccines can be used as prophylaxis and immunotherapy strategies. The aim of this study was to design a multiple-epitope candidate vaccine using leishmaniolysin (GP63) and rK39 proteins against Leishmania major and L. infantum for monocyte-derived exosome preparation. Methods: This study was carried out in Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran, 2023–2024. Effective immunodominant epitopes were selected from two antigenic proteins of GP63 and rK39 using various immunoinformatics and bioinformatics approaches. Vibrio cholerae β-subunit was used as an adjuvant to stimulate immune responses. Then, appropriate linkers were selected for the fusion of epitopes. The 3D model of candidate vaccine was predicted and validated. Results: This designed candidate vaccine could effectively be used as a prophylaxis strategy against leishmaniosis. Conclusion: A candidate vaccine was designed using bioinformatic and immunoinformatic studies with virtual acceptable quality; however, effectiveness of this vaccine should be verified through further in-vitro and in-vivo studies.
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نویسندگان مقاله | Ali Moazezi Ghavihelm
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


| Sedigheh Nabian
Department of Parasitology, School of Veterinary Medicine, University of Tehran, Tehran, Iran


| Shahram Jamshidi
Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran


| Mohammad Taheri
Rastegar Reference Laboratory, School of Veterinary Medicine, University of Tehran, Tehran, Iran


| Minoo Soltani
Rastegar Reference Laboratory, School of Veterinary Medicine, University of Tehran, Tehran, Iran


| Ramin Mazaheri Nezhad Fard
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Ali Akbari Pazoki
Department of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
نشانی اینترنتی https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3984
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