| چکیده انگلیسی مقاله |
Background: Cryptosporidium and Giardia are well-known important intestinal zoonotic pathogens that can infect various hosts and cause diarrhoeal diseases. We aimed to determine the epidemiological prevalence and molecular characterization of Cryptosporidium and Giardia species in Himalayan marmot (Marmota himalayana, class Marmota) in the Qinghai Tibetan Plateau Area of Qinghai Province, Northwest China.
Methods: Overall, 243 Himalayan marmot fecal samples were collected in 2017 and in 2019 and a two-step nested PCR technique was performed to amplify the fragments of the SSU rRNA gene of Cryptosporidium and 18S ribosomal RNA gene of Giardia. Molecular characterization of Cryptosporidium was performed with the primary primers N-DIAGF2 and N-DIAGR2, the secondary primers CPB-DIAGF and CPB-DIAGR. Similarly, molecular characterization of Giardia was used the first primers Gia2029 and Gia2150c, the secondary primers RH11 and RH4. The positive PCR products were sequenced and the sequences were processed by Clustal Omega and BLAST. Phylogenetic analysis was achieved by NJ method in MEGA.
Results: The infection rate of Cryptosporidium spp. and G. duodenalis was 4.9% (12/243) and 0.8% (2/243) in M. himalayana, respectively. Cryptosporidium spp. are characterized as novel genotypes Cryptosporidium marmot genotype I (n=3) and Cryptosporidium marmot genotype II (n=9); G. duodenalis assemblage A (n=2) was found in M. himalayana.
Conclusion: This is the first report of Cryptosporidium spp. and G. duodenalis infections in M. himalayana in Qinghai of Northwest China. The results indicate the existence of Cryptosporidium species and G. duodenalis infections that may have a potential public health significance. |
| نویسندگان مقاله |
| Liqing Ma
Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, 810016, P. R. China
| Yingna Jian
Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, 810016, P. R. China
| Guanghua Wang
Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, 810016, P. R. China
| Qigang Cai
Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, 810016, P. R. China
| Geping Wang
Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, 810016, P. R. China
| Xiuping Li
Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, 810016, P. R. China
| Xueyong Zhang
Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, 810016, P. R. China
| Panagiotis Karanis
1. Qinghai Academy of Animal Sciences and Veterinary Medicine, Qinghai University, Qinghai Provincial Key Laboratory of Pathogen Diagnosis for Animal Disease and Green Technical Research for Prevention and Control, Xining, 810016, P. R. China 2. Medical Faculty and University Hospital Cologne, University of Cologne, Cologne, Cologne, Germany 3. University of Nicosia Medical School, Department of Basic and Clinical Sciences, Anatomy Centre, Nicosia, Cyprus
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