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Iranian Journal of Microbiology، جلد ۱۶، شماره ۳، صفحات ۴۰۱-۴۱۰
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عنوان انگلیسی Designing the fusion protein of rotavirus VP8 and hepatitis A virus VP1 and evaluating the immunological response in BALB/c mice
چکیده انگلیسی مقاله Background and Objectives: Rotavirus and Hepatitis A virus are responsible for causing gastroenteritis and jaundice. The current vaccination approaches have proven insufficient, especially in low-income countries. In this study, we presented a novel dual-vaccine candidate that combines the rotavirus VP8 protein and the hepatitis A virus VP1. Materials and Methods: The VP8*-rotavirus+AAY+HAV-VP1 fusion protein was produced using an Escherichia coli expression system. The recombinant protein had a molecular weight of approximately 45.5 kDa and was purified through affinity chromatography. BALB/c mice were injected subcutaneously with the recombinant protein, VP1, VP8 and vaccines for rotavirus and hepatitis A virus, both with and without ALUM and M720 adjuvants. ELISA assays were used to measure total IgG, IgG1, IgG2, and short-term and long-term IL-5 and IFN-γ responses. Results: The fusion protein, when combined with adjuvants, elicited significantly higher total IgG, IgG1, and IgG2 responses compared to VP1 and VP8 alone, as well as the rotavirus and hepatitis A vaccines. Furthermore, it induced a higher short-term IL-5 and IFN-γ response while demonstrating a higher long-term IL-5 response compared to the rotavirus and hepatitis A vaccines. Conclusion: This study demonstrates that the VP8*-rotavirus+AAY+HAV-VP1 fusion protein is a promising dual vaccine candidate for immunization against hepatitis A and rotaviruses.
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نویسندگان مقاله | Hassan Yarmohammadi
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran; Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran


| Mohammadreza Aghasadeghi
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran


| Abbas Akhavan Sepahi
Department of Microbiology, North Tehran Branch, Islamic Azad University, Tehran, Iran


| Mojtaba Hamidi-fard
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran


| Golnaz Bahramali
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/4600
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