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Iranian Journal of Nuclear Medicine، جلد ۲۳، شماره ۲، صفحات ۷۳-۸۱
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عنوان انگلیسی Synthesis and experimental study of norfloxacin labeled with technecium-99m as a potential agent for infection imaging
چکیده انگلیسی مقاله Introduction: Differentiation between infection and sterile inflammation is one of the most difficult medical problems and is relevant in many clinical situations. Scintigraphy with radiolabeled antibiotics, еspecially labeled fluoroquinolones, is a promising tool for diagnosing bacterial inflammation. We have attempted to synthesize 99mTc-norfloxacin and studied its properties in experiment. Methods: In present study we labeled norfloxacin with 99mTc, determined its radiochemical purity by thin-layer chromatography procedure, compared the bactericidal activity of 99mTc-norfloxacin and unlabelled norfloxacin by disk diffusion, performed in vitro binding assays and evaluated the feasibility of 99mTc-norfloxacin to image soft-tissue infections in rat modal of thigh muscle infection. Scintigraphy was performed at 30 min, 90 min and 18 h post injection. Abscess –to–normal-site ratios were calculated. Results: In this study 99mTc-norfloxacin with high radiochemical purity and low colloid content was synthesized. Disk diffusion method showed that 99mTc-norfloxacin retains bactericidal properties of unlabelled norfloxacin. In vitro binding studies demonstrated small degree of the tracer binding with S. aureus and ability of 99mTc-norfloxacin to bind with killed as well as alive bacteria. Uptake of the tracer was clearly visible in infected muscles and not visible in sterile inflammed muscles. Conclusion: The 99mTc-norfloxacin retains bactericidal properties of unlabelled norfloxacin. The tracer demonstrated small degree of S. aureus binding both with killed as well as alive bacteria. Studies which were performed in vivo in rats with a model of thigh muscle infection showed good ability of the radiopharmaceutical to image the infection.
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نویسندگان مقاله سوتلانا ivanovna sazonova | svetlana ivanovna
research institute of cardiology of the siberian branch of the russian academy of medical sciences, tomsk, russia and national research tomsk polytechnic university, tomsk, russia 3siberian state medical university, tomsk, russia

yuriy borisovich lishmanov | yuriy borisovich
research institute of cardiology of the siberian branch of the russian academy of medical sciences, tomsk, russia

ناتالیا valerievna varlamova | natalia valerievna
national research tomsk polytechnic university, tomsk, russia

viktor sergeevich skuridin | viktor sergeevich
national research tomsk polytechnic university, tomsk, russia

yulia nikolayevna ilushenkova | yulia nikolayevna
research institute of cardiology of the siberian branch of the russian academy of medical sciences, tomsk, russia

ماریا rostislavovna karpova | maria rostislavovna
siberian state medical university, tomsk, russia

yevgeniy alexandrovich nesterov | yevgeniy alexandrovich
national research tomsk polytechnic university, tomsk, russia

نشانی اینترنتی http://irjnm.tums.ac.ir/article_13271_46d5272f969a0f2c6908ddd2e8a31657.pdf
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