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Iranian Journal of Nuclear Medicine، جلد ۱۸، شماره ۲، صفحات ۴۵-۵۱
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عنوان انگلیسی Freeze-dried cold kit for preparation of 99mtc-ciprofloxacin as an infection imaging agent
چکیده انگلیسی مقاله Introduction: Radiolabeled antibiotics are being used for the specific diagnosis of infection by exploiting their specific binding properties to the bacterial components, thereby making it possible to differentiate infection from sterile lesions. The aim of this work was to prepare and evaluate a freeze-dried kit of ciprofloxacin designed for the labeling with 99mTc. Methods: Factors like amount of reducing agent and optimum pH were investigated to make the ciprofloxacin kit. The kit was reconstituted with 99mTc at room temperature and the radiochemical purity was evaluated by ITLC method. Stability and protein binding in human serum followed by in vitro binding to bacteria were assessed. Biodistribution of labeled kit in staphylococcus aureus infected rats muscles were studied using ex vivo counting and scintigraphy. Results: Labeling yield of >90% was obtained corresponding to a specific activity of 178 GBq/mmol. The stability of radiolabeled kit in human serum was 84.2% after 1 hour post incubation. In-vitro studies showed 75 % of radioactivity was bound to bacteria. After injection into mice clearance from the circulation occurred mainly by biliary-renal clearance and site of infection was rapidly detected within 30 min. Target to non-target muscle ratio was 3.23 ± 0.05 % at 30 min post injection. Conclusion: 99mTc-ciprofloxacin showed favorable radiochemical and biological characteristics which permitted detection of the infection with optimal visualization.
کلیدواژه‌های انگلیسی مقاله 99mTc- ciprofloxacin,Infection imaging,Kit preparation
نویسندگان مقاله سیده فاطمه میرشجاعی | seyedeh fatemeh
nuclear science research school, nuclear science and technology research institute, atomic energy organization of iran, tehran, iran
سازمان اصلی تایید شده: سازمان انرژی اتمی (Atomic energy organization)

مصطفی عرفانی |
nuclear science research school, nuclear science and technology research institute, atomic energy organization of iran, tehran, iran
سازمان اصلی تایید شده: سازمان انرژی اتمی (Atomic energy organization)

سید اسماعیل سادات ابراهیمی | seye esmaeil sadat ebrahimi
department of medicinal chemistry, faculty of pharmacy, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد حسین طالبی | mohammad hossein
nuclear science research school, nuclear science and technology research institute, atomic energy organization of iran, tehran, iran
سازمان اصلی تایید شده: سازمان انرژی اتمی (Atomic energy organization)

فرهاد حاج حسن عباسی | haj hassan abbasi
nuclear science research school, nuclear science and technology research institute, atomic energy organization of iran, tehran, iran
سازمان اصلی تایید شده: سازمان انرژی اتمی (Atomic energy organization)

نشانی اینترنتی http://irjnm.tums.ac.ir/article_563_e30d60f6a84c7bea088f6e2c9f36629c.pdf
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