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مجله علوم اعصاب شفای خاتم، جلد ۲، شماره ۴، صفحات ۱۲۲-۱۲۲
عنوان فارسی P۷۲: Bioactive Substance ATC Can Generate Neurosphere from Adipose-Derived Stem Cells (ADSCs)
چکیده فارسی مقاله لطفاً به چکیده انگلیسی مراجعه شود.
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عنوان انگلیسی P72: Bioactive Substance ATC Can Generate Neurosphere from Adipose-Derived Stem Cells (ADSCs)
چکیده انگلیسی مقاله Generation neural stem cells from neurosphere-derived adipose tissue using bioactive substance ATC. Adipose tissue from rat hypodermal and pararenal fat was digested with collagenase, followed by filter and centrifugation the isolated adipose stromal cells were cultured in dishes. These cells evaluated by specific markers of adipose-derived stem cells (ADSCs) such as bioactive substance ATC and then ADSCs differentionated in to neurosphere in four groups that compared morphologically. Diameter and number of this neurosphere evaluated every day in four groups. ADSCs are mesenchymal stem cells that can be extracted from adipose tissue and obtained by a less invasive method. ADSCs markers were measured by immunocytochemistery that expressed CD90 (80%), CD44 (70%) and fibronectin while CD45 didn’t express. Diameter and number of Neurosphere by (0.1 ng/ml) was optimal dose for expansion. Then these cells evaluated by neuroectodermal markers such as nestin and NF 68 that expressed>80% and this data approved by RT-PCR technique. This study develops a simplified, efficient, and nontoxic approach by lowest factors which derives a large number of neurospheres from Adipose-derived stem cells (ADSCs). With our newly devised approach 10 to 15 passage cells were used for in vitro differentiation. Neuronal differentiation was induced by incubation of the ADSCs with bioactive substance (ATC) induction media.
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نویسندگان مقاله لیلا علیزاده | leila alizadeh
shefa neuroscience research center, khatam alanbia hospital, tehran, iran.

سازمان اصلی تایید شده: مرکز تحقیقات علوم اعصاب شفا (Shefa neuroscience research center)

نشانی اینترنتی http://shefayekhatam.ir/browse.php?a_code=A-10-24-470&slc_lang=fa&sid=fa
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/337/article-337-332319.pdf
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده تحقیقات پایه در علوم اعصاب
نوع مقاله منتشر شده پژوهشی
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