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Journal of Basic Research in Medical Sciences، جلد ۴، شماره ۳، صفحات ۴۲-۴۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Detection and enumeration of Cryptosporidium oocysts in environmental water samples by Real-time PCR assay
چکیده انگلیسی مقاله Introduction: The protozoan parasite, Cryptosporidium Spp., widely spreads in both raw and drinking waters. It is the causative agents of waterborne diarrhea and gastroenteritis in the world. In the present study, a molecular assay was used for the detection and quantification of Cryptosporidium oocysts in environmental water samples. Materials and methods: Thirty surface water samples were collected from Rasht City rivers and lagoons during 2009-2010. The samples were analysed for Cryptosporidium oocysts using Real Time PCR method. Samples were filtrated through a 1.2µm pore size cellulose nitrate membrane filter and then purified and quantified by Real-time PCR technique. Results: Cryptosporidium oocysts were found in 19 of 30 the samples. Oocyst concentration was ranging from 0.007 to 27 oocysts per liter of the examined waters. Conclusion: The present study showed that the investigated water supplies were contaminated by Cryptosporidium oocyst. This study indicated that in this level of oocysts there is a potential risk of waterborne cryptosporidiosis due to direct or indirect consumption of these waters by humans and animals. Real-time PCR is a technique that provides high sensitivity for detection quantitative purposes.
کلیدواژه‌های انگلیسی مقاله Cryptosporidium spp., Water, Iran, Real-time PCR
نویسندگان مقاله محمدرضا محمودی | mohammad reza mahmoudi
department of parasitology, faculty of medicine, guilan university of medical sciences, rasht, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی گیلان (Guilan university of medical sciences)

مژگان بنده پور | mojgan bandepour
cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

بهرام کاظمی | bahram kazemi
department of biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

اسد میرزایی | asad mirzaei
department of parasitology, faculty of allied medical sciences, ilam university of medical sciences, ilam, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایلام (Ilam university of medical sciences)

نشانی اینترنتی http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-58-2&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/358/article-358-332463.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Parasitologiy
نوع مقاله منتشر شده پژوهشی
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