| چکیده انگلیسی مقاله |
Background: Avian trichomoniasis is an important disease that causes bird mortality, both wild and captive, around the world. This study evaluated the in vitro cytotoxicity, antioxidant, and antiparasitic activity of citral (3.7-Dimetil-2.6-octadienal) and geraniol (trans-3.7-Dimetil-2.6-octadien-1-ol) against Trichomonas gallinae trophozoites. Methods: In vitro assays were conducted at the Laboratory of Protozoology and Entomology (LAPEN) at the Federal University of Pelotas (UFPel), Brazil in 2019 using tests with 106 parasites and citral and geraniol at concentrations ranging from 10 to 100 μM and four controls: NC (culture medium and trophozoites), MTZ (trophozoites plus 100 μM of metronidazole), and TW (trophozoites plus vehicles used for solubilizing derivatives (0.01% Tween). Results: The citral (60 μM) and geraniol (50 μM) concentrations reduced the trophozoitesʼs viability by 100%. The molecular docking experiment demonstrated that citral and geraniol might inhibit a hydrogen enzyme for T. gallinae survival. Conclusion: The major compounds of lemongrass have potential antitrichomonal activity against T. gallinae in vitro. |
| نویسندگان مقاله |
| Bruna Baccega
Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil
| Juliana Monteli Fenalti
Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil
| Alexia Brauner de Mello
Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil
| Yan Wahast Islabão
Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil
| Paloma Taborda Birmann
Neurobiotechnology Research Group, Postgraduate Program in Biotechnology, Technological Development Center, UFPel, Brazil
| Alessandra Neis
Postgraduate Program in Biotechnology, Technological Development Center, UFPel, Brazil
| Marjorie de Giacomti
Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil
| Francielle Liz Monteiro
Laboratory of Virology and Immunology, Faculty of Veterinary, UFPel, Brazil
| Silvia de Oliveira Hubner
Laboratory of Virology and Immunology, Faculty of Veterinary, UFPel, Brazil
| Rodrigo de Almeida Vaucher
Research Laboratory in Biochemistry and Molecular Biology of Microorganisms (LaPeBBioM), UFPel, Brazil
| Nara Amélia da Rosa Farias
Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil
| Roghayeh Norouzi
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
| Abolghasem Siyadatpanah
Infectious Diseases Research Center, Gonabad University of Medical Sciences, Gonabad, Iran
| Camila Belmonte Oliveira
Parasitology Laboratory, Department of Microbiology and Parasitology, Federal University of Pelotas, Pelotas, Brazil
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