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International Journal of Hematology-Oncology and Stem Cell Research، جلد ۱۹، شماره ۱، صفحات ۱۷-۲۸
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عنوان انگلیسی Innovating Glioma Therapy Using Secretions from Umbilical Cord Mesenchymal Stem Cells to Target Homeobox and Growth Factor Genes
چکیده انگلیسی مقاله Background: Glioblastoma is a prevalent and challenging malignant brain tumor. Secretome therapy using human umbilical cord mesenchymal stem cells (hUCMSCs) appears to be a promising treatment for glioblastoma. This study analyzed the potential of the hUCMSC secretomes (hUCMSCs-sec) for glioma therapy. Materials and Methods: Characterization of hUCMSCs was performed by examining certain markers, including CD44, CD90, CD105, CD73, CD13, CD19, CD14, CD45, CD34, and HLA-D. The cells' ability to differentiate into adipocytes, chondrocytes, and osteocytes was evaluated. Cytotoxic effect on Glioblastoma (GBM) cells was analyzed using 2-[2-methoxy-4-nitrophenyl]-3-[4-nitrophenyl]-5-[2,4-disulfophenyl]-2H-tetrazolium (WST-8). mRNA relative expression, including homeobox (HOXA5, HOXB1, HOXC9 and HOXC10), insulin-like growth factor binding protein 2 (IGFBP2), Extracellular signal-regulated kinases (ERK), Epidermal growth factor receptor (EGFR), and Caspase 3 (Casp3), were quantified by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR). Results: The hUCMSCs-sec was successfully isolated and identified, showing positive markers and its capacity to differentiate into chondrocytes, adipocytes, and osteocytes. hUCMSCs-sec exerted a cytotoxic effect on GBM cells and upregulated the expression of Casp3, whereas it decreased the expression of HOX, IGFBP2, EGFR, and ERK in GBM cells. Conclusion: The secretomes from hUCMSCs show potential for GBM cell therapy by improving the deregulation of HOX, inducing apoptosis, and inhibiting cell proliferation genes.
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نویسندگان مقاله | Ahmad Faried
Department of Neurosurgery, Faculty of Medicine, Padjadjaran University, Bandung 40161, West Java, Indonesia


| Achmad Adam
1)Department of Neurosurgery, Faculty of Medicine, Padjadjaran University, Bandung 40161, West Java, Indonesia 2)Oncology and Stem Cell Working Group, Dr. Hasan Sadikin Hospital, Bandung 40161, West Java, Indonesia


| Wahyu Widowati
Faculty of Medicine, Maranatha Christian University, Bandung 40164, Indonesia


| Annisa Sutendi
Biomolecular and Biomedicine Research Center, Aretha Medika Utama, Bandung 40163, Indonesia


| Faradhina Nindya
Biomolecular and Biomedicine Research Center, Aretha Medika Utama, Bandung 40163, Indonesia


| William Saputro
Biology Study Program, Faculty of Mathematics and Natural Sciences Education, Universitas Pendidikan Indonesia, Bandung 40154, Indonesia


| Dhanar Hadiprasetyo
Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Cimahi 40531, West Java, Indonesia
نشانی اینترنتی https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/2207
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