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Iranian Journal of Parasitology، جلد ۱۲، شماره ۱، صفحات ۸۳-۸۹
عنوان فارسی
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عنوان انگلیسی Genetic Variation of Giardia lamblia Isolates from Food-handlers in Kashan, Central Iran
چکیده انگلیسی مقاله Background: Based on genotyping study of human isolates of Giardia lamblia ; humans are mainly infected by two assemblages A and B. The present study was carried out to determine the sub-assemblages of G. lamblia isolated from food handlers referred to Kashan health centers, central Iran, 2015. Methods: In this cross-sectional study, 3653 stool samples collected from food-handlers that annually refer to health center for getting a health certification and examined microscopically for G. lamblia cyst. Totally, 44 isolates were selected from 47 Giardia positive samples. Cysts were partially purified by the sucrose density gradient method. After freeze-thaw cycles, genomic DNA was extracted using QIAamp Stool Mini kit. A single step PCR-RFLP method was used to amplify a 458bp fragment at the glutamate dehydrogenase (gdh ) locus, restriction enzymes BspLI and RsaI were used for distinguish between genotypes A and B and their subgroups. Results: Of 44 isolates, 24(54.5%) were sub-assemblage AII, 9(20.5%) group B including 7(15.9%) BIII and 2(4.6%) BIV sub-assemblage and 11(25%) isolates showed a mixed pattern of AII and B. Sub-assemblage AI was not detected in this study. Conclusion: The higher rate of sub-assemblage AII demonstrated an anthroponotic origin of the infection so infected food-handlers could directly transmit this protozoan to consumers via contaminated food and water. For finding of pattern of transmission and distribution of Giardia assemblages and sub-assemblage, more studies in human and animal population in different regions are necessary.
کلیدواژه‌های انگلیسی مقاله Giardia,Genetic variation,Food-handlers,PCR-RFLP,Iran
نویسندگان مقاله حسین هوشیار | hossein hooshyar
dept. of parasitology, school of medicine, kashan university of medical sciences, kashan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)

شهربانو غفاری نسب | shahrbanou ghafarinasab
dept. of parasitology, school of medicine, kashan university of medical sciences, kashan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)

محسن اربابی | mohsen arbabi
dept. of parasitology, school of medicine, kashan university of medical sciences, kashan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)

مهدی دلاوری | mahdi delavari
dept. of parasitology, school of medicine, kashan university of medical sciences, kashan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)

سیما راستی | sima rasti
dept. of parasitology, school of medicine, kashan university of medical sciences, kashan, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کاشان (Kashan university of medical sciences)

نشانی اینترنتی http://ijpa.tums.ac.ir/index.php/ijpa/article/view/1521
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/69/article-69-349951.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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